PLK1 [p Ser482, p Ser486, p Ser490] Antibody - Azide Free

Western Blot: PLK1 [p Ser482, p Ser486, p Ser490] Antibody [NB300-278] - Analysis of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase.

• Reactivity: Rt, Xp, Hu, Mu, Rt, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

PLK1 [p Ser482, p Ser486, p Ser490] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 conjugated to KLH. Accession # P70032
• Modification: p Ser482, p Ser486, p Ser490
• Localization: Nuclear
• Marker: Mitosis Marker
• Specificity: Specific for endogenous levels of the ~120k PLK1 protein phosphorylated at Ser482,486,490.
• Predicted Species: Human (100%), Mouse (100%), Rat (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLK1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for PLK1 [p Ser482, p Ser486, p Ser490] Antibody - Azide Free

• cell cycle regulated protein kinase
• EC 2.7.11
• EC 2.7.11.21
• PLK
• PLK1
• PLK-1
• polo (Drosophia)-like kinase
• polo like kinase
• polo-like kinase 1polo-like kinase (Drosophila)
• Serine/threonine-protein kinase 13
• serine/threonine-protein kinase PLK1
• STPK13

Background

Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLK1 Antibody (NB300-278)(3)

Publications using NB300-278

• Erikson E, Haystead TA, Qian YW, Maller JL. A feedback loop in the polo-like kinase activation pathway. Biol Chem 279:32219-32224. 2004-01-01 [PMID: 15166215]
• Liu J, Lewellyn AL, Chen LG, Maller JL. The polo box is required for multiple functions of Plx1 in mitosis. Chem 279:21367-21373. [PMID: 15016807]
• Kumagai A, Dunphy WG. Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts. Science 273:1377-1380. [PMID: 8703070]

Bioinformatics

• Gene Symbol: PLK1
• Uniprot:
  • P53350()
  • P70032(Xenopus)