PLK1 Antibody

Western Blot: PLK1 Antibody [NB100-56651] - Analysis of HCT-116 lysate using PLK1 antibody at 1:2000.

Immunohistochemistry-Paraffin: PLK1 Antibody [NB100-56651] - Analysis of human placenta using PLK1 antibody at 1:100.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PLK1 Antibody Summary

• Description: Novus Biologicals Rabbit PLK1 Antibody (NB100-56651) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PLK1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 100-200 of human PLK1 was used as the immunogen.
• Marker: Mitosis Marker
• Predicted Species: Mouse (97%), Rat (98%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLK1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 28474672)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-1:2000
• Control:

  HCT116 Cell Lysate

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera with PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for PLK1 Antibody

• cell cycle regulated protein kinase
• EC 2.7.11
• EC 2.7.11.21
• PLK
• PLK1
• PLK-1
• polo (Drosophia)-like kinase
• polo like kinase
• polo-like kinase 1polo-like kinase (Drosophila)
• Serine/threonine-protein kinase 13
• serine/threonine-protein kinase PLK1
• STPK13

Background

Polo related kinase (PLK), a serine/threonine kinase is strongly correlated with the mitotic activity of cells. PLK is present in all tissues during mouse embryonic development, where as in adults expresion is limited to thymus and ovaries. It has been observed that tumors of various origin (lung, colon, stomach, smooth muscle, and esophagus as well as non-Hodgkin lymphomas) express high levels of PLK transcripts with absent of transcripts in normal surrounding tissues. Only normal colon and placenta, which are known to be mitotically active express PLK. PLK expression is restricted to proliferating cells. In mammalian cells, three PLKs (PLK1, PLK2/Snk, PLK3/Fnk) have been identified. PLK1 is the most studied isoform. The known substrates of PLK1 include several proteins involved in mitosis such as Cdc25C, APC, and cyclin B. PLK1 promotes mitosis through the phosphorylation and activation of Cdc25C, which regulates the tyrosine dephosphorylation of CDKs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLK1 Antibody (NB100-56651)(1)

Publication using NB100-56651

• Morris Edward J, Kawamura Eiko, Gillespie Jordan A et al. Stat3 regulates centrosome clustering in cancer cells via Stathmin/PLK1. Nature Communications 2017-05-05 [PMID: 28474672] (IF, WB, Human)

Bioinformatics

• Gene Symbol: PLK1
• Entrez:
  • 18817(Mouse)
  • 25515(Rat)
• Uniprot:
  • P53350()