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PLK1 Antibody

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Western Blot: PLK1 Antibody [NB100-56651] - Analysis of HCT-116 lysate using PLK1 antibody at 1:2000.
Western Blot: PLK1 Antibody [NB100-56651] - Analysis of HCT-116 lysate using PLK1 antibody at 1:2000.
Immunohistochemistry-Paraffin: PLK1 Antibody [NB100-56651] - Analysis of human placenta using PLK1 antibody at 1:100.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PLK1 Antibody Summary

Immunogen
A portion of amino acids 100-200 of human PLK1 was used as the immunogen.
Marker
Mitosis Marker
Predicted Species
Mouse (97%), Rat (98%), Bovine (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLK1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Use in ICC/IF was reported in scientific literature (PMID: 28474672).
Control
HCT116 Cell Lysate
Publications
Read Publication using
NB100-56651 in the following applications:

  • IF
    1 publication
  • WB
    1 publication

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (87%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera with PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for PLK1 Antibody

  • cell cycle regulated protein kinase
  • EC 2.7.11
  • EC 2.7.11.21
  • PLK
  • PLK1
  • PLK-1
  • polo (Drosophia)-like kinase
  • polo like kinase
  • polo-like kinase 1polo-like kinase (Drosophila)
  • Serine/threonine-protein kinase 13
  • serine/threonine-protein kinase PLK1
  • STPK13

Background

Polo related kinase (PLK), a serine/threonine kinase is strongly correlated with the mitotic activity of cells. PLK is present in all tissues during mouse embryonic development, where as in adults expresion is limited to thymus and ovaries. It has been observed that tumors of various origin (lung, colon, stomach, smooth muscle, and esophagus as well as non-Hodgkin lymphomas) express high levels of PLK transcripts with absent of transcripts in normal surrounding tissues. Only normal colon and placenta, which are known to be mitotically active express PLK. PLK expression is restricted to proliferating cells. In mammalian cells, three PLKs (PLK1, PLK2/Snk, PLK3/Fnk) have been identified. PLK1 is the most studied isoform. The known substrates of PLK1 include several proteins involved in mitosis such as Cdc25C, APC, and cyclin B. PLK1 promotes mitosis through the phosphorylation and activation of Cdc25C, which regulates the tyrosine dephosphorylation of CDKs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues. Corresponding CDK1 RNA-seq data are presented for the same tissues.
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Cyclin B1 Antibody
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NBP2-44687
Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - MCF-7 cells stained with CF555 Cyclin B1 Antibody (CCNB1/1098) (nuclei/nucleoli, red) and CF488A phalloidin (actin filaments, green). DNA is stained with Hoechst (blue).
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Tonsil stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Endometrial Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)
(1 Review)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P
p53 Antibody (PAb
240)
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
(3 Reviews)
(28 Publications)
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
CDC25C Antibody
NBP2-89147
Western Blot: CDC25C Antibody [NBP2-89147] - Analysis of Cdc25C on human skin tissue lysate using anti-Cdc25C antibody at 1/500 dilution.
Immunocytochemistry/Immunofluorescence: CDC25C Antibody [NBP2-89147] - Staining Cdc25C in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CDC25C Antibody [NBP2-89147] - Analysis of paraffin-embedded human placenta tissue using anti-Cdc25C antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(14 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
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NBP2-32530
Western Blot: PLK3 Antibody [NBP2-32530] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: PLK3 Antibody [NBP2-32530] - Staining in human gallbladder and duodenum tissues using anti-PLK3 antibody. Corresponding PLK3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PLK3 Antibody [NBP2-32530] - Staining of human duodenum shows low expression as expected.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
DNA Polymerase
delta, catalytic subu ...
NBP2-33466
Western Blot: DNA Polymerase delta, catalytic subunit Antibody [NBP2-33466] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: DNA Polymerase delta, catalytic subunit Antibody [NBP2-33466] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: DNA Polymerase delta, catalytic subunit Antibody [NBP2-33466] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
PLK2 Antibody
NBP2-15078
Western Blot: PLK2 Antibody [NBP2-15078] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: PLK2 Antibody [NBP2-15078] - Mouse colon, dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Aurora A Antibody
NBP1-51843
Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti- (NBP1-51843) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution overnight at 4C and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Simple Western: Aurora A Antibody [NBP1-51843] - Simple Western lane view shows a specific band for Aurora A in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.
Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - IF Confocal analysis of HeLa cells using Aurora A antibody (NBP1-51843, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
(1 Review)
(6 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Chk1 Antibody
NB100-464
Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of Chk1 show equal loading of the pairs of lanes. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/19084), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
(8 Publications)
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APC Antibody
NB100-91662
Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate. Lane2:HEK293T cell lysate. Lane3:Rat testis tissue lysate.
Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Coagulation Factor
XIV/Protein C Antibod ...
NBP2-66987
Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Analysis of paraffin-embedded human liver tissue using anti-Protein C antibody. Counter stained with hematoxylin.
Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
PCNA Antibody
(PC10)
NB500-106
Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. Immunofluorescence for PCNA (yellow) and DAPI staining (blue) on 30 um-cryosections of E32-P1 tree shrew neocortex. The top margin of the image corresponds to the transition zone SVZ/intermediate zone. Scale bars, 50 um. VZ, ventricular zone; SVZ, subventricular zone; iSVZ, inner SVZ; oSVZ, outer SVZ. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fnana.2018.00029/full), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization.
(5 Reviews)
(33 Publications)
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
PLK4 Antibody
NB100-894
Western Blot: PLK4 Antibody [NB100-894] - Analysis of PLK4 in human Colon lysate (35ug protein in RIPA buffer) using NB100-894 at 2 ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunocytochemistry/Immunofluorescence: PLK4 Antibody [NB100-894] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: PLK4 Antibody [NB100-894] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
(3 Publications)
Species: Hu
Applications: WB, Flow, ICC/IF, PEP-ELISA
Aurora B Antibody
NB100-294
Immunocytochemistry/Immunofluorescence: Aurora B Antibody [NB100-294] - RPE1 cells stained with Aurora B antibody (green). Image from verified customer review.
Immunohistochemistry: Aurora B Antibody [NB100-294] - Rabbit polyclonal to Aurora B (NB100-294) used to stain SW620 human tumour xenografts (in mouse). The sections were microwave pretreated in citrate buffer (pH 6.0) for 5 mins high then 5 mins simmer (800W conventional microwave). Slides were then incubated for 1 hour with the Aurora B primary antibody diluted 1/200 in TBS, then visualised using DAB, after application of an appropriate secondary.
Chromatin Immunoprecipitation: Aurora B Antibody [NB100-294] - 293T cells stained with Aurora B antibody. Image from verified customer review.
(5 Reviews)
(11 Publications)
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, MiAr
FoxM1 Antibody
NBP1-30961
Western Blot: FoxM1 Antibody [NBP1-30961] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody [C3], C-term diluted at 1:5000.
Immunohistochemistry-Paraffin: FoxM1 Antibody [NBP1-30961] - Representative images of immunoreactivity of nuclear FOXM1 in (I) benign cystadenoma, (II) borderline tumour, (III) stage I invasive cancer, (IV) stage II invasive cancer, (V) stage III invasive cancer and (VI) stage IV invasive cancer. Magnifications X400. Insets: Regions with higher magnifications of nuclear FOXM1 staining. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0113478), licensed under a CC-BY licence.
(1 Review)
(2 Publications)
Species: Hu
Applications: WB, ChIP, IHC, IHC-P
TCEAL1 Antibody
(OTI2G10)
NBP2-46085
Western Blot: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TCEAL1.
Immunohistochemistry: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(
Immunohistochemistry: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Species: Hu
Applications: WB, IHC
D4S234E Antibody
(1C3)
H00027065-M01
Western Blot: D4S234E Antibody (1C3) [H00027065-M01] - D4S234E monoclonal antibody (M01), clone 1C3 Analysis of D4S234E expression in IMR-32.
Western Blot: D4S234E Antibody (1C3) [H00027065-M01] - D4S234E monoclonal antibody (M01), clone 1C3. Analysis of D4S234E expression in PC-12.
Western Blot: D4S234E Antibody (1C3) [H00027065-M01] - D4S234E monoclonal antibody (M01), clone 1C3. Analysis of D4S234E expression in rat brain.
(1 Publication)
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
CDC5L Antibody
(JJ091-05)
NBP2-67901
Western Blot: CDC5L Antibody (JJ091-05) [NBP2-67901] - Analysis of CDC5L on different lysates using anti-CDC5L antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat
Immunocytochemistry/Immunofluorescence: CDC5L Antibody (JJ091-05) [NBP2-67901] - Staining CDC5L in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CDC5L Antibody (JJ091-05) [NBP2-67901] - Analysis of paraffin-embedded mouse lung tissue using anti-CDC5L antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
PLK1 Antibody
NB100-56651
PLK1 Antibody
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P
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Publications for PLK1 Antibody (NB100-56651)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IF, WB.


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Product General Protocols

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Control Lysate(s)

HCT116 Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLK1
Entrez
Uniprot