PLK1 Antibody (AZ24)

Images

 
Western Blot: Polo like kinase Antibody (AZ24) [NB100-2792] -Pix in HeLa whole cell extracts detected with NB100-2746 (Lane 1) and NB100-2792 (Lane 2).

Product Details

Summary
Reactivity Hu, Mu, XpSpecies Glossary
Applications WB, ELISA, IP
Clone
AZ24
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

PLK1 Antibody (AZ24) Summary

Immunogen
Baculo expressed Polo Kinase from Xenopus laevis
Localization
Nuclear
Marker
Mitosis Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PLK1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunoprecipitation 1:10 - 1:500
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Notes

Positive control(s): Hela cells.

Alternate Names for PLK1 Antibody (AZ24)

  • cell cycle regulated protein kinase
  • EC 2.7.11
  • EC 2.7.11.21
  • PLK
  • PLK1
  • PLK-1
  • polo (Drosophia)-like kinase
  • polo like kinase
  • polo-like kinase 1polo-like kinase (Drosophila)
  • Serine/threonine-protein kinase 13
  • serine/threonine-protein kinase PLK1
  • STPK13

Background

Polo-like kinase (PLK) plays an important role in cell cycle regulation. It is required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a polo-like kinase kinase (PLKK) that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLK1
Entrez