PLK1 [p Thr210] Antibody (8G9X10)

Western Blot: PLK1 [p Thr210] Antibody (8G9X10) [NBP3-16917] - Western blot analysis of extracts of HT-29 cells, using PLK1 (NBP3-16917) at 1:1000 dilution.HT-29 cells were treated by nocodazole (100 ng/mL) at 37C for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

• Reactivity: Hu
• Applications: WB
• Clone: 8G9X10
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PLK1 [p Thr210] Antibody (8G9X10) Summary

• Description: Novus Biologicals Rabbit PLK1 [p Thr210] Antibody (8G9X10) (NBP3-16917) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic phosphorylated peptide around T210 of human PLK1 (P53350). KKTLC
• Modification: p Thr210
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PLK1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PLK1 [p Thr210] Antibody (8G9X10)

• cell cycle regulated protein kinase
• EC 2.7.11
• EC 2.7.11.21
• PLK
• PLK1
• PLK-1
• polo (Drosophia)-like kinase
• polo like kinase
• polo-like kinase 1polo-like kinase (Drosophila)
• Serine/threonine-protein kinase 13
• serine/threonine-protein kinase PLK1
• STPK13

Background

Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLK1