PINX1 Antibody - BSA Free

Western Blot: PINX1 Antibody [NBP2-32265] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PINX1 antibody NBP2-32265 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry: PINX1 Antibody [NBP2-32265] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PINX1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Immunoprecipitation: PINX1 Antibody [NBP2-32265] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-PINX1 antibody NBP2-32265 used for IP at 6 ug per reaction. PINX1 was also immunoprecipitated by rabbit anti-PINX1 antibody BL15294. For blotting immunoprecipitated PINX1, NBP2-32265 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PINX1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PINX1 Antibody - BSA Free (NBP2-32265) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-PINX1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 225 to 275 of human PIN2 Interacting Protein 1 using the numbering given in entry NP_060354.4 (GeneID 54984).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PINX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:1000 - 1:5000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PINX1 Antibody - BSA Free

• FLJ20565
• hepatocellular carcinoma-related putative tumor suppressor
• Liver-related putative tumor suppressor
• LPTLPinX1
• LPTS67-11-3 protein
• MGC8850
• PIN2 interacting protein 1
• PIN2/TERF1 interacting, telomerase inhibitor 1
• PIN2/TERF1-interacting telomerase inhibitor 1
• PIN2-interacting protein 1
• Pin2-interacting protein X1
• Protein 67-11-3
• TRF1-interacting protein 1

Background

PINX1 is a protein that contains a TID (telomerase inhibiting domain) domain which is capable of binding MCRS1, TERT and TERF1. PINX1 has been shown to be a potent telomerase inhibitor and putative tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PINX1 Antibody (NBP2-32265)(2)

Publications using NBP2-32265

• Hou P, Li H, Yong H et al. PinX1 represses renal cancer angiogenesis via the mir-125a-3p/VEGF signaling pathway Angiogenesis 2019-06-28 [PMID: 31254127]
• Jiang, T, Li, H Et al. Pin2/TRF1 binding protein X1 inhibits colorectal cancer cell migration and invasion in vitro and metastasis in vivo via the nuclear factor kappa B signaling pathway. Oncol Rep 2018-09-01 [PMID: 30015978] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: PINX1
• Uniprot:
  • NP_060354.4(Human)