MCRS1 Antibody - Azide and BSA Free

Western Blot: MCRS1 Antibody [H00010445-B01P] - Western blot analysis of MCRS1 expression in whole testis extracts from 4.5, 6.5, 9.5, 12.5, and 15.5 dpp and adult mice. Each lane was loaded with 50 ug of testis protein extracts. Alpha tubulin was used as internal control. Image collected and cropped by CiteAb from the following publication (//link.springer.com/article/10.1007/s00412-017-0631-z) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: MCRS1 Antibody [H00010445-B01P] - Analysis of purified antibody to MCRS1 on HeLa cell. (antibody concentration 10 ug/ml)

Immunohistochemistry-Paraffin: MCRS1 Antibody [H00010445-B01P] - Analysis of purified antibody to MCRS1 on formalin-fixed paraffin-embedded human spleen. (antibody concentration 3 ug/ml)

Western Blot: MCRS1 Antibody [H00010445-B01P] - Analysis of MCRS1 expression in transfected 293T cell line by MCRS1 polyclonal antibody. Lane 1: MCRS1 transfected lysate(50.82 KDa). Lane 2: Non-transfected lysate.

Western Blot: MCRS1 Antibody [H00010445-B01P] - Identification of interacting partners of PRDM9 by Y2H screening. a Schematic representation of the domain structure of seven PRDM9-interacting proteins identified by Y2H screening. The prey fragments identified in the screens are shown as blue bars underneath each protein. The length (number of amino acid residues) of each full-length protein is indicated on the right. b RNA expression of the PRDM9-interacting proteins in mouse testes (left) & ovaries (right) during meiosis. Data were extracted from RNA-seq data in Margolin et al. (2014) & Soh et al. (2015)). The average gene expression is shown in reads per kilobase per million of reads (RPKM) & fragments per kilobase of transcript per million mapped read (FKPM) for testes & ovaries, respectively. Zoomed-in views of the dashed line boxes are shown below. Spo11 & Spata19 expression were used as positive & negative controls, respectively. c Western blot analysis of PRDM9, CXXC1, PIH1D1, CEP70, & MCRS1 expression in whole testis extracts from 4.5, 6.5, 9.5, 12.5, & 15.5 dpp & adult mice. Each lane was loaded with 50 μg of testis protein extracts. Tubulin alpha was used as internal control Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28527011), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MCRS1 Antibody - Azide and BSA Free Summary

• Immunogen: MCRS1 (NP_006328.2, 1 a.a. - 462 a.a.) full-length human protein. MDKDSQGLLDSSLMASGTASRSEDEESLAGQKRASSQALGTIPKRRSSSRFIKRKKFDDELVESSLAKSSTRAKGASGVEPGRCSGSEPSSSEKKKVSKAPSTPVPPSPAPAPGLTKRVKKSKQPLQVTKDLGRWKPADDLLLINAVLQTNDLTSVHLGVKFSCRFTLREVQERWYALLYDPVISKLACQAMRQLHPEAIAAIQSKALFSKAEEQLLSKVGSTSQPTLETFQDLLHRHPDAFYLARTAKALQAHWQLMKQYYLLEDQTVQPLPKGDQVLNFSDAEDLIDDSKLKDMRDEVLEHELMVADRRQKREIRQLEQELHKWQVLVDSITGMSSPDFDNQTLAVLRGRMVRYLMRSREITLGRATKDNQIDVDLSLEGPAWKISRKQGVIKLKNNGDFFIANEGRRPIYIDGRPVLCGSKWRLSNNSVVEIASLRFVFLINQDLIALIRAEAAKITPQ
• Specificity: MCRS1 - microspherule protein 1,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: MCRS1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. May also be used for immunofluorescence, immunohistochemistry (paraffin).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MCRS1 Antibody - Azide and BSA Free

• Cell cycle-regulated factor p78
• ICP22BP
• INO80 complex subunit Q
• INO80Q58 kDa microspherule protein
• MCRS2INO80 complex subunit J
• microspherule protein 1
• MSP58cell cycle-regulated factor (78 kDa)
• P78

Background

Modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. May be an inhibitor of TERT telomerase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCRS1 Antibody (H00010445-B01P)(3)

Publications using H00010445-B01P

• Imai Y, Baudat F, Taillepierre M et al. The PRDM9 KRAB domain is required for meiosis and involved in protein interactions. Chromosoma 2017-05-19 [PMID: 28527011]
• Zhong M, Zhang X, Li B et al. Expression of MSP58 in hepatocellular carcinoma. Med Oncol. 2013-03-22 [PMID: 23519485]
• Xu CS, Zheng JY, Zhang HL et al. MSP58 Knockdown Inhibits the Proliferation of Esophageal Squamous Cell Carcinoma in Vitro and in Vivo. Asian Pac J Cancer Prev. 2012-11-01 [PMID: 22994740]

Bioinformatics

• Gene Symbol: MCRS1
• Entrez:
  • 10445(Human)
• Uniprot:
  • NP_006328.2(Human)