TFIP11 Antibody

Western Blot: TFIP11 Antibody [NBP1-40364] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TFIP11 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. TFIP11 was also immunoprecipitated by rabbit anti-TFIP11 antibody NBP1-40365 which recognizes a downstream epitope.

Immunohistochemistry: TFIP11 Antibody [NBP1-40364] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- TFIP11 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt, Bv, Ca, Pm, Pm, Pm-Or, Rb
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TFIP11 Antibody Summary

• Description: Novus Biologicals Rabbit TFIP11 Antibody (NBP1-40364) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 1 and 50 of human tuftelin interacting protein 11 using the numbering given in entry NP_036275.1 (GeneID 24144).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Monkey (100%), Bovine (100%), Orangutan (100%), Rabbit (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TFIP11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TFIP11 Antibody

• bK445C9.6
• DKFZP434B194
• FLJ22086
• hNtr1
• NTR1
• Septin and tuftelin-interacting protein 1
• STIP
• STIP-1
• TIP39Spp382
• tuftelin interacting protein 11
• tuftelin-interacting protein 11

Background

Neurotensin (NT) initiates an intracellular response by interacting with the G protein-coupled receptors NTR1 (NTS1 receptor, high affinity NTR) and NTR2 (NTS2 receptor, levocabastine-sensitive neurotensin receptor), and the type I receptor NTR3 (NTS3 receptor, sortilin-1, Gp95). NT has a wide distribution in regions of the brain and in peripheral tissues where NT receptors can contribute to hypotension, hyperglycemia, hypothermia, antinociception and regulation of intestinal motility and secretion. HL-60 cells express NTR1, which can couple to Gq, Gi/o, or Gs. Alternative splicing of rat NTR2 can generate a 5-transmembrane domain variant isoform that is co-expressed with the full-length NTR2 throughout the brain and spinal cord. NTR3 activation in the murine microglial cell line N11 induces MIP-2, MCP-1, IL-1beta and TNFalpha in an ERK1/2 and Akt kinase-dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TFIP11
• Entrez:
  • 24144(Human)