PIMT Antibody

Images

 
Western Blot: PIMT Antibody [NB100-2595] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-2595 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and ...read more
Immunohistochemistry: PIMT Antibody [NB100-2595] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PIMT used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: PIMT Antibody [NB100-2595] - FFPE section of human non-small cell lung cancer. Affinity purified rabbit anti-PIMT used at a dilution of 1:250.
Independent Antibodies: Immunoprecipitation: PIMT Antibody [NB100-2595] - Detection of human PIMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

PIMT Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human PRIP-Interacting Protein with Methyltransferase Domain using the numbering given in entry NP_079107.5 (GeneID 96764).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TGS1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIMT Antibody

  • Cap-specific guanine-N2 methyltransferase
  • CLL-associated antigen KW-2
  • DKFZp762A163
  • EC 2.1.1
  • EC 2.1.1.-
  • HCA137
  • Hepatocellular carcinoma-associated antigen 137
  • NCOA6IPSEREX-defined
  • nuclear receptor coactivator 6 interacting protein
  • Nuclear receptor coactivator 6-interacting protein
  • PIMTFLJ22995
  • PIPMT
  • PRIP-interacting protein PIPMT
  • PRIP-interacting protein with methyltransferase domain
  • PRIP-interacting protein with methyltransferase motif
  • trimethylguanosine synthase 1
  • trimethylguanosine synthase homolog (S. cerevisiae)
  • trimethylguanosine synthase homolog
  • trimethylguanosine synthase

Background

Nuclear receptor coactivators participate in the transcriptional activation of specific genes by nuclear receptors. A new nuclear receptor coactivator-interacting protein, designated PIMT, was identified from a human liver cDNA library by using the coactivator peroxisome proliferator-activated receptor-interacting protein (PRIP) as bait in a yeast 2-hybrid screen. The PIMT cDNA encodes an 852-amino acid protein containing a 9-amino acid methyltransferase motif I (VVDAFCGVG) and an invariant segment (GXXGXXI) found in K-homology motifs of many RNA-binding proteins. Northern blot analysis demonstrated ubiquitous expression of a 3.2-kb PIMT transcript, with highest expression in heart, skeletal muscle, kidney, liver, and placenta. Immunofluorescence studies showed that the PIMT and PRIP proteins are colocalized in the nucleus. PIMT binds 5-adenosyl-L-methionine, the methyl donor for the methyltransfer reaction, and it also binds RNA, suggesting that it is an RNA methyltransferase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TGS1
Entrez
Uniprot