NOP58 Antibody

Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-46846] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NOP58 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: NOP58 Antibody [NBP1-46846] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NOP58 antibody NBP1-46846 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. NOP58 was also immunoprecipitated by rabbit anti-NOP58 antibody NBP1-46845, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

NOP58 was stabilized by MIR4435-2HG and mediated the pro-tumorigenetic role of MIR4435-2HG in HCC. A The expression of NOP58 mRNA in HCC cells with MIR4435-2HG interference in HCC cells. B The expression of NOP58 protein in HCC cells with MIR4435-2HG overexpressed in HCC cells. C Western blot analysis detected the expression of NOP58 protein in HCC cells with treatment of CHX. D Western blot analysis detected the expression of NOP58 protein in HCC cells with treatment of MG132. E Expression analysis of NOP58 protein in HCC based on CPTAC database. F Representative images of the expression of NOP58 protein in paired tumor and nontumor tissues by IHC. G The relationship between MIR4435-2HG expression and NOP58 expression in 20 HCC samples. H Knockdown of NOP58 by siRNA in HCC cells. I Cellular proliferation of HCC cells was detected by CCK8 assay. J Cellular proliferation of HCC cells was detected by EdU assay. K Colony formation ability of HCC cells was detected. L The spheroids form ability of HCC cells. M Expression of stem cell markers were detected in the indicated groups by qPCR. The data are shown as mean +/- SD. *P < 0.05, **P < 0.01, ***P < 0.001. White scale bar, 100 μm Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37891494), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

NOP58 was stabilized by MIR4435-2HG and mediated the pro-tumorigenetic role of MIR4435-2HG in HCC. A The expression of NOP58 mRNA in HCC cells with MIR4435-2HG interference in HCC cells. B The expression of NOP58 protein in HCC cells with MIR4435-2HG overexpressed in HCC cells. C Western blot analysis detected the expression of NOP58 protein in HCC cells with treatment of CHX. D Western blot analysis detected the expression of NOP58 protein in HCC cells with treatment of MG132. E Expression analysis of NOP58 protein in HCC based on CPTAC database. F Representative images of the expression of NOP58 protein in paired tumor and nontumor tissues by IHC. G The relationship between MIR4435-2HG expression and NOP58 expression in 20 HCC samples. H Knockdown of NOP58 by siRNA in HCC cells. I Cellular proliferation of HCC cells was detected by CCK8 assay. J Cellular proliferation of HCC cells was detected by EdU assay. K Colony formation ability of HCC cells was detected. L The spheroids form ability of HCC cells. M Expression of stem cell markers were detected in the indicated groups by qPCR. The data are shown as mean +/- SD. *P < 0.05, **P < 0.01, ***P < 0.001. White scale bar, 100 μm Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37891494), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

NOP58 was stabilized by MIR4435-2HG and mediated the pro-tumorigenetic role of MIR4435-2HG in HCC. A The expression of NOP58 mRNA in HCC cells with MIR4435-2HG interference in HCC cells. B The expression of NOP58 protein in HCC cells with MIR4435-2HG overexpressed in HCC cells. C Western blot analysis detected the expression of NOP58 protein in HCC cells with treatment of CHX. D Western blot analysis detected the expression of NOP58 protein in HCC cells with treatment of MG132. E Expression analysis of NOP58 protein in HCC based on CPTAC database. F Representative images of the expression of NOP58 protein in paired tumor and nontumor tissues by IHC. G The relationship between MIR4435-2HG expression and NOP58 expression in 20 HCC samples. H Knockdown of NOP58 by siRNA in HCC cells. I Cellular proliferation of HCC cells was detected by CCK8 assay. J Cellular proliferation of HCC cells was detected by EdU assay. K Colony formation ability of HCC cells was detected. L The spheroids form ability of HCC cells. M Expression of stem cell markers were detected in the indicated groups by qPCR. The data are shown as mean +/- SD. *P < 0.05, **P < 0.01, ***P < 0.001. White scale bar, 100 μm Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37891494), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

NOP58 was stabilized by MIR4435-2HG and mediated the pro-tumorigenetic role of MIR4435-2HG in HCC. A The expression of NOP58 mRNA in HCC cells with MIR4435-2HG interference in HCC cells. B The expression of NOP58 protein in HCC cells with MIR4435-2HG overexpressed in HCC cells. C Western blot analysis detected the expression of NOP58 protein in HCC cells with treatment of CHX. D Western blot analysis detected the expression of NOP58 protein in HCC cells with treatment of MG132. E Expression analysis of NOP58 protein in HCC based on CPTAC database. F Representative images of the expression of NOP58 protein in paired tumor and nontumor tissues by IHC. G The relationship between MIR4435-2HG expression and NOP58 expression in 20 HCC samples. H Knockdown of NOP58 by siRNA in HCC cells. I Cellular proliferation of HCC cells was detected by CCK8 assay. J Cellular proliferation of HCC cells was detected by EdU assay. K Colony formation ability of HCC cells was detected. L The spheroids form ability of HCC cells. M Expression of stem cell markers were detected in the indicated groups by qPCR. The data are shown as mean +/- SD. *P < 0.05, **P < 0.01, ***P < 0.001. White scale bar, 100 μm Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37891494), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

NOP58 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 479 and 529 of human NOP58 ribonucleoprotein homolog using the numbering given in entry NP_057018.1 (GeneID 51602).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOP58
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200- 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NOP58 Antibody

• HSPC120
• NOL5
• NOP5/NOP58
• NOP58 ribonucleoprotein homolog (yeast)
• NOP5nucleolar protein 58
• Nucleolar protein 5
• nucleolar protein NOP5/NOP58

Background

Nop5p (a.k.a. Nop58p), is localized in the nucleolus and required for pre-18S rRNA processing in Saccharomyces cerevisiae (baker's yeast).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NOP58 Antibody (NBP1-46846)(1)

Publication using NBP1-46846

• Zhu Y, Xiao B, Liu M et al. N6-methyladenosine-modified oncofetal lncRNA MIR4435-2HG contributed to stemness features of hepatocellular carcinoma cells by regulating rRNA 2'-O methylation Cellular & molecular biology letters 2023-10-27 [PMID: 37891494] (WB, IP, IHC, Human)
  
  Details:
  WB Dilution 1:1000 ,IHC Dilution 1:200

Bioinformatics

• Gene Symbol: NOP58