PIK3CA Antibodies
We offer PIK3CA Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each PIK3CA Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PIK3CA Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the PIK3CA Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PIK3CA polyclonal antibodies and browse our PIK3CA monoclonal antibody catalog.
Alternate Names for PIK3CA Antibodies
anti-PIK3CA antibody, anti-EC 2.7.1 antibody, anti-EC 2.7.1.153 antibody, anti-MGC142161 antibody, anti-MGC142163 antibody, anti-p110-alpha antibody, anti-Phosphatidylinositol 3-Kinase catalytic subunit alpha antibody, anti-phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha antibody, anti-phosphatidylinositol 3-kinase, catalytic, alpha polypeptide antibody, anti-Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody, anti-phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody, anti-phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform antibody, anti-phosphoinositide-3-kinase, catalytic, alpha polypeptide antibody, anti-PI3-kinase p110 subunit alpha antibody, anti-PI3-kinase subunit alpha antibody, anti-PI3K antibody, anti-PI3K-alpha antibody, anti-PtdIns-3-kinase p110 antibody, anti-PtdIns-3-kinase subunit alpha antibody, anti-PtdIns-3-kinase subunit p110-alpha antibody
16 Results for "pik3ca" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: PIK3CA Antibody [NBP2-19804] - HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PI3 kinase p110 alpha protein stained by PI3 kinase p110 alpha antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19804-img0009.jpg "Immunocytochemistry/Immunofluorescence: PIK3CA Antibody [NBP2-19804] - HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PI3 kinase p110 alpha protein stained by PI3 kinase p110 alpha antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP2-19804] - Human Breast Cancer, using PI3 kinase p110 alpha antibody. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Immunohistochemistry-Paraffin-NBP2-19804-img0004.jpg)
![Western Blot: PIK3CA Antibody [NBP2-19804] - PIK3CA Antibody Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM NBP2-19804 diluted at 1:1000.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Western-Blot-NBP2-19804-img0006.jpg)
![Western Blot: PIK3CA Antibody [NBP2-19804] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Western-Blot-NBP2-19804-img0007.jpg)
![Western Blot: PIK3CA Antibody [NBP2-19804] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PIK3CA antibody (NBP2-19804) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-19804_rabbit-polyclonal-pik3ca-antibody-910202420134031.jpg)
![Immunocytochemistry/ Immunofluorescence: PIK3CA Antibody [NBP3-35070] - Immunofluorescence analysis of U2OS cells using PIK3CA Rabbit pAb (A0265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35070_rabbit-pik3ca-pab-10122024121045.jpg "Immunocytochemistry/ Immunofluorescence: PIK3CA Antibody [NBP3-35070] - Immunofluorescence analysis of U2OS cells using PIK3CA Rabbit pAb (A0265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: PIK3CA Antibody [NBP3-35070] - Western Blot analysis of various lysates using PIK3CA Rabbit pAb at 1:5000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-35070_rabbit-pik3ca-pab-3112025173116.jpg)
![Immunocytochemistry/ Immunofluorescence: PIK3CA Antibody [NBP3-35070] - Immunofluorescence analysis of NIH/3T3 cells using PIK3CA Rabbit pAb (A0265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35070_rabbit-pik3ca-pab-101220241224136.jpg)
![Immunocytochemistry/ Immunofluorescence: PIK3CA Antibody [NBP3-35070] - Immunofluorescence analysis of PC-12 cells using PIK3CA Rabbit pAb (A0265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35070_rabbit-pik3ca-pab-1012202411572694.jpg)
![Immunohistochemistry: PIK3CA Antibody [NBP3-35070] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PIK3CA Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35070_rabbit-pik3ca-pab-101220241233847.jpg)
![Immunohistochemistry: PIK3CA Antibody [NBP3-35070] - Immunohistochemistry analysis of paraffin-embedded Rat liver using PIK3CA Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35070_rabbit-pik3ca-pab-101220241243934.jpg)
![Immunohistochemistry: PIK3CA Antibody [NBP3-35070] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PIK3CA Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35070_rabbit-pik3ca-pab-31120251744431.jpg)
![Immunohistochemistry: PIK3CA Antibody [NBP3-35070] - Immunohistochemistry analysis of paraffin-embedded Rat liver using PIK3CA Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35070_rabbit-pik3ca-pab-31120251704726.jpg)
![Immunohistochemistry: PIK3CA Antibody [NBP3-35070] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using PIK3CA Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35070_rabbit-pik3ca-pab-311202517705.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0011.jpg "Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0001.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0002.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0003.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0004.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0005.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0006.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0007.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0008.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0009.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0010.jpg)
![Western Blot: PIK3CA Antibody (4F3) [NBP2-37511] - Western blot analysis using PIK3CA mAb against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-4F3-Western-Blot-NBP2-37511-img0003.jpg "Western Blot: PIK3CA Antibody (4F3) [NBP2-37511] - Western blot analysis using PIK3CA mAb against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: PIK3CA Antibody (4F3) [NBP2-37511] - Immunofluorescence analysis of HeLa cells using PIK3CA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-4F3-Immunocytochemistry-Immunofluorescence-NBP2-37511-img0004.jpg)
![Flow Cytometry: PIK3CA Antibody (4F3) [NBP2-37511] - Flow cytometric analysis of Jurkat cells using PIK3CA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PIK3CA-Antibody-4F3-Flow-Cytometry-NBP2-37511-img0005.jpg)
![Western Blot: PIK3CA Antibody (4F3) [NBP2-37511] - Western blot analysis using PIK3CA mAb against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-4F3-Western-Blot-NBP2-37511-img0002.jpg)
![ELISA: PIK3CA Antibody (4F3) [NBP2-37511] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/PIK3CA-Antibody-4F3-ELISA-NBP2-37511-img0001.jpg)
![Immunohistochemistry-Paraffin: PIK3CA Antibody (CL8347) [NBP2-88915] - Staining of human stomach cancer shows moderate cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-CL8347-Immunohistochemistry-Paraffin-NBP2-88915-img0005.jpg "Immunohistochemistry-Paraffin: PIK3CA Antibody (CL8347) [NBP2-88915] - Staining of human stomach cancer shows moderate cytoplasmic positivity in tumor cells.")
![Immunohistochemistry-Paraffin: PIK3CA Antibody (CL8347) [NBP2-88915] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-CL8347-Immunohistochemistry-Paraffin-NBP2-88915-img0001.jpg)
![Immunohistochemistry-Paraffin: PIK3CA Antibody (CL8347) [NBP2-88915] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-CL8347-Immunohistochemistry-Paraffin-NBP2-88915-img0002.jpg)
![Immunohistochemistry-Paraffin: PIK3CA Antibody (CL8347) [NBP2-88915] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-CL8347-Immunohistochemistry-Paraffin-NBP2-88915-img0003.jpg)
![Immunohistochemistry-Paraffin: PIK3CA Antibody (CL8347) [NBP2-88915] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-CL8347-Immunohistochemistry-Paraffin-NBP2-88915-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PIK3CA Antibody (CL8347) [NBP2-88915] - Analysis in human placenta and skeletal muscle tissues using NBP2-88915 antibody. Corresponding PIK3CA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-CL8347-Immunohistochemistry-Paraffin-NBP2-88915-img0006.jpg)
![Western Blot: PIK3CA Antibody [NBP3-37884] - Western blot analysis of various lysates using PIK3CA Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-37884_rabbit-pik3ca-pab-1012202411545581.jpg "Western Blot: PIK3CA Antibody [NBP3-37884] - Western blot analysis of various lysates using PIK3CA Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: PIK3CA Antibody [NBP3-37884] - Western blot analysis of lysates from 293T cells, using PIK3CA Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-37884_rabbit-pik3ca-pab-1012202411572692.jpg)
![Immunohistochemistry: PIK3CA Antibody [NBP3-37884] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using PIK3CA Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37884_rabbit-pik3ca-pab-3112025173150.jpg)
![Immunohistochemistry: PIK3CA Antibody [NBP3-37884] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using PIK3CA Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37884_rabbit-pik3ca-pab-311202516593622.jpg)
![Immunohistochemistry-Paraffin: PIK3CA Antibody [NB100-55408] - Staining of paraffin embedded human heart with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Immunohistochemistry-Paraffin-NB100-55408-img0002.jpg "Immunohistochemistry-Paraffin: PIK3CA Antibody [NB100-55408] - Staining of paraffin embedded human heart with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: PIK3CA Antibody [NB100-55408] - Staining of paraffin embedded human brain (cerebellar cortex) with antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Immunohistochemistry-Paraffin-NB100-55408-img0001.jpg)
![Western Blot: PIK3CA Antibody (10E5) [NBP3-26384] - Positive Western Blot detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate. All lanes: PIK3CA Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 125 kDa Observed band size: 110 kDa](http://images.novusbio.com/fullsize/nbp3-26384_rabbit-pik3ca-mab-10e5-282202411442616.jpg "Western Blot: PIK3CA Antibody (10E5) [NBP3-26384] - Positive Western Blot detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate. All lanes: PIK3CA Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 125 kDa Observed band size: 110 kDa")
![Immunocytochemistry/Immunofluorescence: PIK3CA Antibody (10E5) [NBP3-26384] - Staining of Hela Cells with PIK3CA Antibody (10E5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26384_rabbit-pik3ca-mab-10e5-282202412493610.jpg)
![Western Blot: PIK3CA Antibody [NBP2-98814] - Lane A: Mouse heart tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:124 kDaObserved band size:90 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Western-Blot-NBP2-98814-img0002.jpg "Western Blot: PIK3CA Antibody [NBP2-98814] - Lane A: Mouse heart tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:124 kDaObserved band size:90 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: PIK3CA Antibody [NBP2-98814] - Immunofluorescence staining of PIK3CA in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human PIK3CA polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98814-img0001.jpg)
![Proximity Ligation Assay: PIK3CA Antibody (3G3) [H00005290-M01] - Analysis of protein-protein interactions between APPL1 and PIK3CA. HeLa cells were stained with anti-APPL1 rabbit purified polyclonal 1:1200 and anti-PIK3CA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PIK3CA-Antibody-3G3-Proximity-Ligation-Assay-H00005290-M01-img0002.jpg "Proximity Ligation Assay: PIK3CA Antibody (3G3) [H00005290-M01] - Analysis of protein-protein interactions between APPL1 and PIK3CA. HeLa cells were stained with anti-APPL1 rabbit purified polyclonal 1:1200 and anti-PIK3CA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Sandwich ELISA: PIK3CA Antibody (3G3) [H00005290-M01] - Detection limit for recombinant GST tagged PIK3CA is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-3G3-Sandwich-ELISA-H00005290-M01-img0003.jpg)
![Western Blot: PIK3CA Antibody [NBP3-35708] - Western Blot analysis of extracts of 293T cells, using PIK3CA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35708_rabbit-pik3ca-pab-31120251744484.jpg%3Ft=1698885985 "Western Blot: PIK3CA Antibody [NBP3-35708] - Western Blot analysis of extracts of 293T cells, using PIK3CA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
