PHD4/HIF Prolyl Hydroxylase 4 Antibody [HRP]

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Product Details

Summary
Reactivity Hu, Rt, Mu(-)Species Glossary
Applications WB, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

PHD4/HIF Prolyl Hydroxylase 4 Antibody [HRP] Summary

Immunogen
The epitope recognized by this antibody maps to a region between residue 450 and the C-terminus (residue 502) of human PHD4/HIF Prolyl Hydroxylase 4 using the numbering given in entry NP_808808.1 (GeneID 54681).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
P4HTM
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry-Paraffin
Application Notes
This PHD4/HIF Prolyl Hydroxylase 4 antibody is useful for Western Blot where a band is seen at ~50 kDa. Use in Immunocytochemistry/immunofluorescence, Immunohistochemistry-Paraffin and Electron Microscopy reported in scientific literature (PMID 17003483)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System

Reactivity Notes

Does not appear to work in Mouse. Rat reactivity reported in scientific literature (PMID: 17003483)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PHD4/HIF Prolyl Hydroxylase 4 Antibody [HRP]

  • EC 1.14.11.-
  • EGLN4
  • HIFPH4
  • HIF-PH4
  • HIF-prolyl hydroxylase 4
  • HPH-4
  • hypoxia-inducible factor prolyl 4-hydroxylase
  • Hypoxia-inducible factor prolyl hydroxylase 4
  • P4H with transmembrane domain
  • P4H-TMFLJ20262
  • PH4 hypoxia inducible factor prolyl 4 hydroxylase
  • PH-4
  • PHD4
  • proline 4-hydroxylase
  • prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
  • Prolyl hydroxlase domain-containing 4
  • transmembrane prolyl 4-hydroxylase

Background

Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. Prolyl hydroxylase 4, specifically, catalyzes the post-translational formation of 4-hydroxyproline in HIF alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, may targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol P4HTM