PGK2 Antibody

Images

 
Western Blot: PGK2 Antibody [NBP1-31452] - Sample(30 ug of whole cell lysate) A:293T B:HeLa S3 7.5% SDS PAGE diluted at 1:1000.
Immunohistochemistry-Paraffin: PGK2 Antibody [NBP1-31452] - Paraffin-embedded mixed OVCA, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.19 mg/ml

Order Details

PGK2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Phosphoglycerate kinase 2. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Primate (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PGK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (84%), Porcine (87%), Rat (85%), Bovine (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.19 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PGK2 Antibody

  • dJ417L20.2
  • EC 2.7.2.3
  • PGK-2
  • PGKB
  • PGKPS
  • phosphoglycerate kinase 2
  • phosphoglycerate kinase autosomal pseudogene
  • Phosphoglycerate kinase, testis specific

Background

The PGK2 gene encodes a testis-specific form of phosphoglycerate kinase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. See also PGK1 (MIM 311800), which is ubiquitously expressed in all somatic tissues and maps to chromosome Xq13.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol PGK2