PGK2 Antibody - BSA Free

Western Blot: PGK2 Antibody [NBP1-31452] - Sample(30 ug of whole cell lysate) A:293T B:HeLa S3 7.5% SDS PAGE diluted at 1:1000.

Immunohistochemistry-Paraffin: PGK2 Antibody [NBP1-31452] - Paraffin-embedded mixed OVCA, using antibody at 1:100 dilution.

• Reactivity: Hu, Pm
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PGK2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PGK2. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Primate (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PGK2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Porcine (87%), Rat (85%), Bovine (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PGK2 Antibody - BSA Free

• dJ417L20.2
• EC 2.7.2.3
• PGK-2
• PGKB
• PGKPS
• phosphoglycerate kinase 2
• phosphoglycerate kinase autosomal pseudogene
• Phosphoglycerate kinase, testis specific

Background

The PGK2 gene encodes a testis-specific form of phosphoglycerate kinase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. See also PGK1 (MIM 311800), which is ubiquitously expressed in all somatic tissues and maps to chromosome Xq13.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PGK2