CRISP-1 Antibody (840017) [Unconjugated]

Western blot shows lysates of mouse epididymis tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse CRISP‑1 Monoclonal Antibody (Catalog # MAB4675) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CRISP‑1 at approximately 27-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CRISP‑1 was detected in perfusion fixed frozen sections of mouse epididymis using Rat Anti-Mouse CRISP‑1 Monoclonal Antibody (Catalog # MAB4675) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Recombinant Mouse CRISP‑1 inhibits proliferation in the 3A‑sub E human placenta cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation inhibited by Recombinant Mouse CRISP‑1 (10 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CRISP‑1 Monoclonal Antibody (Catalog # MAB4675). The ND50 is typically 10-20 µg/mL.

• Reactivity: Mu
• Applications: WB, IHC, B/N
• Clone: 840017
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CRISP-1 Antibody (840017) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CRISP-1
  Gln20-His244
  Accession # Q03401
• Specificity: Detects mouse CRISP-1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Crisp1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 10-20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRISP-1 Antibody (840017) [Unconjugated]

• Acidic epididymal glycoprotein homolog
• acidic epididymal glycoprotein-like 1
• AEGL1
• AEG-related protein
• ARPAEG-like protein
• CRISP1
• CRISP-1
• CRISP-1AEGL1HSCRISP1D
• cysteine-rich secretory protein 1
• cysteine-rich secretory protein-1 delta
• HSCRISP1G
• HUMARP

Background

Cysteine-rich Secretory Protein 1 (CRISP‑1), also known as Acidic Epididymal Glycoprotein 1 (AEG1) and Sperm‑Coating Glycoprotein 1 (SCP1), is a 29-32 kDa (26.4 kDa predicted) protein that is involved in male reproductive biology (1, 2). CRISPs belong to the CAP superfamily of molecules that also includes several snake, insect, and lizard venom proteins (3). Structurally, CRISPs consist of an N-terminal SCP/CAP domain, a hinge region, and a Cys-rich domain with 16 invariant cysteine residues (4). CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction (1). Expression patterns, genomic structure, and sequence conservation indicate that the likely ortholog of human CRISP-1 in mouse and rat is CRISP-4 (5, 6). Mature mouse CRISP-1 shares 68% amino acid (aa) sequence identity with rat CRISP-1. It shares 55%, 73%, and 43% aa sequence identity with mouse CRISP-2, -3, and -4, respectively. Rodent CRISP-1 is secreted by the epididymal epithelium and associates with the surface of spermatozoa in the epididymis where it inhibits capacitation (7-9). It is released from the sperm cell surface once capacitation begins in the female reproductive tract or in vitro (10). CRISP-1 is involved in the interaction of spermatozoa with the oocyte zona pellucida as well as in the fusion of sperm and egg (11, 12). CRISP-1 is additionally expressed in the lower medulla of hair shafts and under androgen control in the submandibular salivary glands of male mice (13-15).

1. Koppers, A.J. et al. (2011) Asian J. Androl. 13:111.
2. Gibbs, G.M. et al. (2008) Endocr. Rev. 29:865.
3. Yamazaki, Y. and T. Morita (2004) Toxicon 44:227.
4. Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
5. Jalkanen, J. et al. (2005) Biol. Reprod. 72:1268.
6. Nolan, M.A. et al. (2006) Biol. Reprod. 74:984.
7. Charest, N.J. et al. (1989) Endocrinology 125:942.
8. Maldera, J.A. et al. (2011) Biol. Reprod. 85:503.
9. Nixon, B. et al. (2006) Biol. Reprod. 74:275.
10. Roberts, K.P. et al. (2003) Biol. Reprod. 69:572.
11. Busso, D. et al. (2007) Biol. Reprod. 77:848.
12. Da Ros, V.G. et al. (2008) Dev. Biol. 320:12.
13. Haendler, B. et al. (1993) Endocrinology 133:192.
14. Mizuki, N. and M. Kasahara (1992) Mol. Cell. Endocrinol. 89:25.
15. Peterson, R.L. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:238.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRISP-1 Antibody (MAB4675)(1)

Publication using MAB4675

• X Zhang, L Wang, Y Ma, Y Wang, H Liu, M Liu, L Qin, J Li, C Jiang, X Zhang, X Shan, Y Liu, J Li, Y Li, R Zheng, Y Sun, J Sun, X Leng, Y Liang, F Zhang, X Jiang, Y Yang, Y Shen CEP128 is involved in spermatogenesis in humans and mice Nature Communications, 2022;13(1):1395. 2022 [PMID: 35296684] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Crisp1
• Uniprot:
  • Q03401()