CRISP-1 Antibody (840017) [Unconjugated]

Images

 
Western blot shows lysates of mouse epididymis tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse CRISP‑1 Monoclonal Antibody (Catalog # MAB4675) followed by HRP-conjugated Anti-Rat IgG Secondary ...read more
CRISP‑1 was detected in perfusion fixed frozen sections of mouse epididymis using Rat Anti-Mouse CRISP‑1 Monoclonal Antibody (Catalog # MAB4675) at 10 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
Recombinant Mouse CRISP‑1 inhibits proliferation in the 3A‑sub E human placenta cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation inhibited by Recombinant ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clone
840017
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

CRISP-1 Antibody (840017) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CRISP-1
Gln20-His244
Accession # Q03401
Specificity
Detects mouse CRISP-1 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
Crisp1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 10-20 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRISP-1 Antibody (840017) [Unconjugated]

  • Acidic epididymal glycoprotein homolog
  • acidic epididymal glycoprotein-like 1
  • AEGL1
  • AEG-related protein
  • ARPAEG-like protein
  • CRISP1
  • CRISP-1
  • CRISP-1AEGL1HSCRISP1D
  • cysteine-rich secretory protein 1
  • cysteine-rich secretory protein-1 delta
  • HSCRISP1G
  • HUMARP

Background

Cysteine-rich Secretory Protein 1 (CRISP‑1), also known as Acidic Epididymal Glycoprotein 1 (AEG1) and Sperm‑Coating Glycoprotein 1 (SCP1), is a 29-32 kDa (26.4 kDa predicted) protein that is involved in male reproductive biology (1, 2). CRISPs belong to the CAP superfamily of molecules that also includes several snake, insect, and lizard venom proteins (3). Structurally, CRISPs consist of an N-terminal SCP/CAP domain, a hinge region, and a Cys-rich domain with 16 invariant cysteine residues (4). CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction (1). Expression patterns, genomic structure, and sequence conservation indicate that the likely ortholog of human CRISP-1 in mouse and rat is CRISP-4 (5, 6). Mature mouse CRISP-1 shares 68% amino acid (aa) sequence identity with rat CRISP-1. It shares 55%, 73%, and 43% aa sequence identity with mouse CRISP-2, -3, and -4, respectively. Rodent CRISP-1 is secreted by the epididymal epithelium and associates with the surface of spermatozoa in the epididymis where it inhibits capacitation (7-9). It is released from the sperm cell surface once capacitation begins in the female reproductive tract or in vitro (10). CRISP-1 is involved in the interaction of spermatozoa with the oocyte zona pellucida as well as in the fusion of sperm and egg (11, 12). CRISP-1 is additionally expressed in the lower medulla of hair shafts and under androgen control in the submandibular salivary glands of male mice (13-15).
  1. Koppers, A.J. et al. (2011) Asian J. Androl. 13:111.
  2. Gibbs, G.M. et al. (2008) Endocr. Rev. 29:865.
  3. Yamazaki, Y. and T. Morita (2004) Toxicon 44:227.
  4. Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
  5. Jalkanen, J. et al. (2005) Biol. Reprod. 72:1268.
  6. Nolan, M.A. et al. (2006) Biol. Reprod. 74:984.
  7. Charest, N.J. et al. (1989) Endocrinology 125:942.
  8. Maldera, J.A. et al. (2011) Biol. Reprod. 85:503.
  9. Nixon, B. et al. (2006) Biol. Reprod. 74:275.
  10. Roberts, K.P. et al. (2003) Biol. Reprod. 69:572.
  11. Busso, D. et al. (2007) Biol. Reprod. 77:848.
  12. Da Ros, V.G. et al. (2008) Dev. Biol. 320:12.
  13. Haendler, B. et al. (1993) Endocrinology 133:192.
  14. Mizuki, N. and M. Kasahara (1992) Mol. Cell. Endocrinol. 89:25.
  15. Peterson, R.L. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:238.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol Crisp1
Uniprot