PGC-1 beta Antibody (6C3F6) - BSA Free

Western Blot: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Western blot analysis using PPARGC1B mAb against HEK293 (1) and PPARGC1B (AA: 195-414)-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Flow cytometric analysis of Hela cells using PPARGC1B mouse mAb (green) and negative control (red).

Western Blot: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Western blot analysis using PPARGC1B mAb against human PPARGC1B (AA: 195-414) recombinant protein. (Expected MW is 50.3 kDa)

ELISA: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 6C3F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PGC-1 beta Antibody (6C3F6) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human PGC-1 beta (AA: 195-414) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPARGC1B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 113.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PGC-1 beta Antibody (6C3F6) - BSA Free

• DKFZp686C1790
• ERRL1
• PERCFLJ14284
• peroxisome proliferator-activated receptor gamma, coactivator 1 beta
• PGC1
• PGC1Bgamma, coactivator 1, beta
• PPARGC1

Background

PGC-1 beta is a tissue specific co-activator that enhances the activity of estrogen receptor alpha. The co-activation function relies on a bipartite transcriptional activation domain and two LXXLL motifs that interact with the AR2 domain of ER-alpha in an estrogen-dependent manner. PGC-1 beta is also involved in activation of nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. Ectopic expression of this protein has been shown to lead to increased mitochondrial numbers and basal oxygen consumption.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPARGC1B