PF1 Antibody [Alexa Fluor® 700]

• Reactivity: Hu, Mu, Ch
• Applications: WB, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 700

PF1 Antibody [Alexa Fluor® 700] Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 954 and 1004 of human PHD factor 1 using the numbering given in entry NP_001028733.1 (GeneID 57649).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHF12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 22986495).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PF1 Antibody [Alexa Fluor® 700]

• FLJ34122
• KIAA1523MGC131914
• PF1
• PHD factor 1
• PHD finger protein 12
• PHD zinc finger transcription factor

Background

PHD factor 1 (PF1) was identified as a protein that interacts with mSin3A-histone deacetylase corepressor, a multiprotein complex that mediates transcriptional repression. PF1 is a plant homeodomain zinc finger protein that also bears a forkhead-associated (FHA) domain. PF1 appears to facilitate the recruitment of the mSin3A complex to DNA for transcriptional repression. It has also been shown to interact with the histone deacetylase containing corepressor transducin-like enhancer of split (TLE) and may play a role in bridging the mSin3A and TLE transcriptional networks. Alternate names for PF1 include PHF12, PHD finger protein 12, and KIAA1523.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PHF12