GRP75/HSPA9B/Mortalin Antibody (OTI9F8)

Western Blot: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of extracts from 9 different cell lines: (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRP75/HSPA9B/Mortalin.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Human pancreas tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.

Flow Cytometry: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of Jurkat cells, using anti-Mortalin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRP75/HSPA9B/Mortalin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRP75/HSPA9B/Mortalin.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Human endometrium tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.

Flow Cytometry: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of Hela cells, using anti-Mortalin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, IHC-P
• Clone: OTI9F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GRP75/HSPA9B/Mortalin Antibody (OTI9F8) Summary

• Immunogen: Full length human recombinant protein of human HSPA9 (NP_004125) produced in HEK293T cell.
• Marker: Immortalization Marker
• Specificity: This antibody is specific for Homo sapiens heat shock 70kDa protein 9 (mortalin) (HSPA9), nuclear gene encoding mitochondrial protein.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000-2000
• Theoretical MW: 73.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GRP75/HSPA9B/Mortalin Antibody (OTI9F8)

• 75 kDa glucose-regulated protein
• CSA
• GRP75
• GRP-75
• GRP75mthsp75
• Heat shock 70 kDa protein 9
• heat shock 70kD protein 9B
• heat shock 70kDa protein 9 (mortalin)
• heat shock 70kDa protein 9B (mortalin-2)
• HSPA9
• HSPA9B
• HSPA9BMGC4500
• Mortalin
• mortalin, perinuclear
• Mortalin-2
• MOT
• MOT2
• mot-2
• MTHSP75
• p66-mortalin
• PBP74
• PBP74CSA
• Peptide-binding protein 74
• stress-70 protein, mitochondrial

Background

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein plays a role in the control of cell proliferation. It may also act as a chaperone

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GRP75/HSPA9B/Mortalin Antibody (NBP1-47801)(1)

Publication using NBP1-47801

• Yan Y, Yu L, Castro L, Dixon D. ERa36, a variant of estrogen receptor a, is predominantly localized in mitochondria of human uterine smooth muscle and leiomyoma cells. PLoS ONE. 2017-10-11 [PMID: 29020039] (WB, Human)

Bioinformatics

• Gene Symbol: HSPA9
• Entrez:
  • 3313(Human)