GRP75/HSPA9B/Mortalin Antibody (OTI9F8)

Images

 
Western Blot: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of extracts from 9 different cell lines: (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: ...read more
Immunocytochemistry/ Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRP75/HSPA9B/Mortalin.
Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Human pancreas tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.
Flow Cytometry: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of Jurkat cells, using anti-Mortalin antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRP75/HSPA9B/Mortalin (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Human endometrium tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.
Flow Cytometry: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of Hela cells, using anti-Mortalin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI9F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GRP75/HSPA9B/Mortalin Antibody (OTI9F8) Summary

Immunogen
Full length human recombinant protein of human HSPA9 (NP_004125) produced in HEK293T cell.
Marker
Immortalization Marker
Specificity
This antibody is specific for Homo sapiens heat shock 70kDa protein 9 (mortalin) (HSPA9), nuclear gene encoding mitochondrial protein.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HSPA9
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Theoretical MW
73.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-47801 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for GRP75/HSPA9B/Mortalin Antibody (OTI9F8)

  • 75 kDa glucose-regulated protein
  • CSA
  • GRP75
  • GRP-75
  • GRP75mthsp75
  • Heat shock 70 kDa protein 9
  • heat shock 70kD protein 9B
  • heat shock 70kDa protein 9 (mortalin)
  • heat shock 70kDa protein 9B (mortalin-2)
  • HSPA9
  • HSPA9B
  • HSPA9BMGC4500
  • Mortalin
  • mortalin, perinuclear
  • Mortalin-2
  • MOT
  • MOT2
  • mot-2
  • MTHSP75
  • p66-mortalin
  • PBP74
  • PBP74CSA
  • Peptide-binding protein 74
  • stress-70 protein, mitochondrial

Background

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein plays a role in the control of cell proliferation. It may also act as a chaperone

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GRP75/HSPA9B/Mortalin Antibody (NBP1-47801)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GRP75/HSPA9B/Mortalin Antibody (NBP1-47801) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70963AF350
Alexa Fluor 405 NBP2-70963AF405
Alexa Fluor 488 NBP2-70963AF488
Alexa Fluor 532 NBP2-70963AF532
Alexa Fluor 594 NBP2-70963AF594
Alexa Fluor 647 NBP2-70963AF647
Alexa Fluor 700 NBP2-70963AF700
Alexa Fluor 750 NBP2-70963AF750
Allophycocyanin NBP2-70963APC
Biotin NBP2-70963B
DyLight 350 NBP2-70963UV
DyLight 405 NBP2-70963V
DyLight 488 NBP2-70963G
DyLight 550 NBP2-70963R
DyLight 594 NBP2-70963DL594
DyLight 650 NBP2-70963C
DyLight 680 NBP2-70963FR
DyLight 755 NBP2-70963IR
FITC NBP2-70963F
HRP NBP2-70963H
Janelia Fluor 549 NBP2-70963JF549
Janelia Fluor 646 NBP2-70963JF646
PE NBP2-70963PE
PerCP NBP2-70963PCP

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Bioinformatics Tool for GRP75/HSPA9B/Mortalin Antibody (NBP1-47801)

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Bioinformatics

Gene Symbol HSPA9
Entrez