PF1 Antibody

Western Blot: PF1 Antibody [NB100-81671] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-PF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 minutes.

Immunoprecipitation: PF1 Antibody [NB100-81671] - Detection of human PF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PF1 antibody NB100-81671 used for IP at 3 ug per reaction. PF1 was also immunoprecipitated by rabbit anti-PF1 antibody NB100-81670. For blotting immunoprecipitated PF1, NB100-81671 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu, Ch
• Applications: WB, ChIP, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

PF1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 954 and 1004 of human PHD factor 1 using the numbering given in entry NP_001028733.1 (GeneID 57649).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHF12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Chip reactivity reported in scientific literature (PMID: 22986495).

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 22986495).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PF1 Antibody

• FLJ34122
• KIAA1523MGC131914
• PF1
• PHD factor 1
• PHD finger protein 12
• PHD zinc finger transcription factor

Background

PHD factor 1 (PF1) was identified as a protein that interacts with mSin3A-histone deacetylase corepressor, a multiprotein complex that mediates transcriptional repression. PF1 is a plant homeodomain zinc finger protein that also bears a forkhead-associated (FHA) domain. PF1 appears to facilitate the recruitment of the mSin3A complex to DNA for transcriptional repression. It has also been shown to interact with the histone deacetylase containing corepressor transducin-like enhancer of split (TLE) and may play a role in bridging the mSin3A and TLE transcriptional networks. Alternate names for PF1 include PHF12, PHD finger protein 12, and KIAA1523.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PF1 Antibody (NB100-81671)(2)

Publications using NB100-81671

• Nishibuchi G, Shibata Y, Hayakawa T et al. Physical and Functional Interactions between the Histone H3K4 Demethylase KDM5A and the Nucleosome Remodeling and Deacetylase (NuRD) Complex. J Biol Chem. 2014 Sep 04 [PMID: 25190814]
• Strobl-Mazzulla PH, Bronner ME. A PHD12-Snail2 repressive complex epigenetically mediates neural crest epithelial-to-mesenchymal transition J Cell Biol 2012 Sep 17 [PMID: 22986495] (ChIP, Chicken)

Bioinformatics

• Gene Symbol: PHF12
• Entrez:
  • 57649(Human)
  • 268448(Mouse)
• Uniprot:
  • Q96QT6(Human)