PF1 Antibody

Images

 
Western Blot: PF1 Antibody [NB100-81671] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-PF1 ...read more
Independent Antibodies: Immunoprecipitation: PF1 Antibody [NB100-81671] - Detection of human PF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from ...read more

Product Details

Summary
Reactivity Hu, Mu, ChSpecies Glossary
Applications WB, ChIP, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

PF1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 954 and 1004 of human PHD factor 1 using the numbering given in entry NP_001028733.1 (GeneID 57649).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PHF12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Chip reactivity reported in scientific literature (PMID: 22986495).
Publications
Read Publications using
NB100-81671 in the following applications:

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 22986495).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PF1 Antibody

  • FLJ34122
  • KIAA1523MGC131914
  • PF1
  • PHD factor 1
  • PHD finger protein 12
  • PHD zinc finger transcription factor

Background

PHD factor 1 (PF1) was identified as a protein that interacts with mSin3A-histone deacetylase corepressor, a multiprotein complex that mediates transcriptional repression. PF1 is a plant homeodomain zinc finger protein that also bears a forkhead-associated (FHA) domain. PF1 appears to facilitate the recruitment of the mSin3A complex to DNA for transcriptional repression. It has also been shown to interact with the histone deacetylase containing corepressor transducin-like enhancer of split (TLE) and may play a role in bridging the mSin3A and TLE transcriptional networks. Alternate names for PF1 include PHF12, PHD finger protein 12, and KIAA1523.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PF1 Antibody (NB100-81671)(2)

We have publications tested in 1 confirmed species: Chicken.

We have publications tested in 1 application: ChIP.


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ChIP
(1)
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Filter By Species
Chicken
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for PF1 Antibody (NB100-81671) (0)

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol PHF12
Entrez
Uniprot