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MRG15 Antibody

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Western Blot: MRG15 Antibody [NB100-1307] - staining of NIH3T3 (A) and HepG2 (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western Blot: MRG15 Antibody [NB100-1307] - staining of NIH3T3 (A) and HepG2 (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Immunohistochemistry-Paraffin: MRG15 Antibody [NB100-1307] - staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data was obtained using a previous batch.
Western Blot: MRG15 Antibody [NB100-1307] - Analysis of Mouse Liver lysate (35ug protein in RIPA buffer) using NB100-1307 at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: MRG15 Antibody [NB100-1307] - staining of Mouse Liver (A), Rat Liver (B) and Pig Liver (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Immunohistochemistry-Paraffin: MRG15 Antibody [NB100-1307] - staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data was obtained using a previous batch.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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MRG15 Antibody Summary

Immunogen
Peptide with sequence APKQDPKPKFQEG-C corresponding to N-Terminus according to NP_006782.1, NP_996670.1.
Specificity
This antibody is expected to recognise isoform 1 (NP_006782.1) and isoform 2 (NP_996670.1).
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MORF4L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 ug/ml
Application Notes
WB: Approx. 38 kDa band observed in mouse and rat liver lysates (calculated MW of 37.2 kDa band according to mouse NP_077751.1). IHC-P: Human uterus shows nuclear staining in the epithelial cells of the endometrial glands. Data obtained from a previous batch (different goat).
Control
MRG15 Overexpression Lysate
Publications
Read Publication using NB100-1307.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MRG15 Antibody

  • Eaf3
  • Esa1p-associated factor 3 homolog
  • HsT17725
  • MEAF3
  • MGC10631
  • MORF-related gene on chromosome 15
  • MORFRG15
  • mortality factor 4 like 1
  • mortality factor 4-like protein 1
  • MRG15MORF-related gene 15 protein
  • Protein MSL3-1
  • S863-6
  • Transcription factor-like protein MRG15

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: TIP60 Antibody [NBP2-24613] - analysis of TIP60 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain lysate using this antibody. 25 ug/ml.
Simple Western: TIP60 Antibody [NBP2-24613] - Simple Western lane view shows a specific band for TIP60 in 0.2 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: HDAC1 Antibody [NB100-56340] - Analysis of HDAC1 in HEK293 cell lysate with this antibody.
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Applications: IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
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Western Blot: MRFAP1 Antibody [NBP1-98552] - Antibody Dilution: 1.0ug/ml Sample Tissue: Jurkat cell lysate.
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Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Representative Western blot showing M-track protein protein proximity signals obtained for Kic1. Hog1-protA-H3: background control, Nup2: positive control. Below: Proximity signals. n = 3 replicates per sample except when indicated differently. Ratios are log2-transformed. Black lines indicate average proximity signal. Proximity signals that differ significantly from background are marked in green (q 0.01) filled circles. Grey filled triangles: q > 0.05. N: N-terminal HKMTmyc fusion. Image collected and cropped by CiteAb from the following publication (https://biosignaling.biomedcentral.com/articles/10.1186/s12964-019-0381-z), licensed under a CC-BY licence.
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 K9-me3 in HeLa histone lysates. Observed molecular weight is ~15 kDa.
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(23 Publications)
Species: Ce, Dr, Hu, In, Ma, Mu, Po, Rt, Ye
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, WB
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Deacetylase 2/HDAC2 A ...
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Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.
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12.5% SDS-PAGE Stained with Coomassie Blue.
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Western Blot: H4/j Overexpression Lysate (Adult Normal) [NBL1-11567] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for H4/j.
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MRG15 Antibody
NB100-1307
MRG15 Antibody
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, IHC, IHC-P, PEP-ELISA

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Control Lysate(s)

MRG15 Overexpression Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MORF4L1
Entrez
Uniprot