Human Periostin/OSF-2 ELISA Kit (Colorimetric)

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ELISA: Human Periostin/OSF-2 ELISA Kit (Colorimetric) [NBP3-18052] - Samples were spiked with high concentrations of Human Periostin/OSF-2 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human Periostin/OSF-2 ELISA Kit (Colorimetric) [NBP3-18052] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16-10 ng/mL
Sensitivity
0.09 ng/mL

Order Details

Human Periostin/OSF-2 ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.16-10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 5.43
Intra-Assay
%CV < 6.30
Spike Recovery
89-108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
POSTN

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Periostin/OSF-2 ELISA Kit (Colorimetric)

  • Fasciclin I-like
  • MGC119510
  • MGC119511
  • OSF2
  • OSF-2
  • OSF-2osteoblast specific factor 2 (fasciclin I-like)
  • OSF2periodontal ligament-specific periostin
  • Osteoblast-specific factor 2
  • PDLPOSTN
  • periostin isoform thy2
  • periostin isoform thy4
  • periostin isoform thy6
  • periostin isoform thy8
  • Periostin
  • periostin, osteoblast specific factor
  • PNRP11-412K4.1
  • POSTN
  • TRIF52

Background

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol POSTN