Perilipin-4 Antibody

Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP1-06072] - (3.8ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Perilipin-4 Antibody Summary

• Immunogen: Peptide with sequence C-RTTDNGGEQTALSPQE corresponding to internal region according to NP_001073869.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PLIN4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:2000
• Application Notes: WB: Preliminary experiments gave bands at approx. 45 kDa band and 50 kDa band in human adipose lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 134 kDa band according to NP_001073869.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Human pancreas shows membranous and cytoplasm staining in adipocytes.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Perilipin-4 Antibody

• Adipocyte protein S3-12
• KIAA1881S3-12
• perilipin 4
• perilipin-4

Background

Perilipin-4 may play a role in triacylglycerol packaging into adipocytes. May function as a coat protein involved in thebiogenesis of lipid droplets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Perilipin-4 Antibody (NBP1-06072)(1)

Publication using NBP1-06072

• Wolins NE, Brasaemle DL, Bickel PE. A proposed model of fat packaging by exchangeable lipid droplet proteins. FEBS Lett 2006-10-09 [PMID: 16962104]

Bioinformatics

• Gene Symbol: PLIN4
• Entrez:
  • 729359(Human)
• Uniprot:
  • Q96Q06(Human)