Perilipin-2/ADFP Antibody

Images

 
Simple Western: Perilipin-2/ADFP Antibody [NB110-40878] - Lane view shows a specific band for ADFP in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody [NB110-40878] - ADFP antibody was tested in mouse liver using DAB with hematoxylin counterstain.
Immunohistochemistry-Frozen: Perilipin-2/ADFP Antibody [NB110-40878] - Detection of PLIN2 in pig sleletal muscle cross section. Antibody dilution 1:100 in PBS with 2% normal goat serum. Secondary antibody: Alexa Fluor ...read more
Western Blot: Perilipin-2/ADFP Antibody [NB110-40878] - Detection of human ADFP in human liver lysate.
Western Blot: Perilipin-2/ADFP Antibody [NB110-40878] - CDK5 and 14-3-3iu and KIAA0528 are found on lipid droplet (LD) cargo. Intact purified LDs were probed with antibodies against CDK5, 14-3-3iu, KIAA0528, and GFP ...read more
Flow Cytometry: Perilipin-2/ADFP Antibody [NB110-40878] - An intracellular stain was performed on HepG2 cells with Perilipin-2 Antibody NB110-40878B (blue) and a matched isotype control (orange). Both antibodies were ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, Pm, PmSpecies Glossary
Applications WB, Simple Western, Flow, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.23 mg/ml

Perilipin-2/ADFP Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of human ADFP (within residues 150-250) [Swiss-Prot# Q99541]
Localization
Membrane, cytoplasm
Predicted Species
Primate (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLIN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/mL
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Simple Western 1:50
  • Western Blot 2 ug/ml
Application Notes
This ADFP antibody is useful for Western blot analysis where a band is observed ~48 kDa, and Immunohistochemistry of paraffin embedded sections where cytoplasmic and membrane staining was observed in mouse liver. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

This Perilipin-2/ADFP Antibody is validated for IHC-Fr from a verified customer review.
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
Perilipin-2/ADFP Overexpression Lysate
Control Peptide
Perilipin-2/ADFP Peptide (NB110-40878PEP)
Reviewed Applications
Read 2 Reviews rated 2.5
using
NB110-40878 in the following applications:

Publications
Read Publications using
NB110-40878 in the following applications:

  • 1 publication
  • WB
    3 publications

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 30651536). Immunogen has 89% identity to porcine and 87% identity to bovine. Porcine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.23 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Perilipin-2/ADFP Antibody

  • ADFP
  • Adipophilin
  • Adipose differentiation-related proteinadipophilin
  • ADRP
  • MGC10598
  • perilipin 2
  • Perilipin2
  • Perilipin-2
  • PLIN2

Background

Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase. ADFP is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to specific cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Perilipin-2/ADFP Antibody (NB110-40878)(3)

Reviews for Perilipin-2/ADFP Antibody (NB110-40878) (2) 2.52

Average Rating: 2.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Bovine and Mouse, Pig.

Reviews using NB110-40878:
Filter by Applications
IHC-Fr
(1)
WB
(1)
All Applications
Filter by Species
Bovine and Mouse
(1)
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Perilipin-2/ADFP NB110-40878
Enlarge
4
reviewed by:
Verified Customer
IHC-Fr Pig 09/15/2020
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSkeletal muscle
SpeciesPig
LotD-1

Comments

Commentsno fluorescence was detected when blocking the antibody binding in advance with blocking peptide
Western Blot Perilipin-2/ADFP NB110-40878
Enlarge
1
reviewed by:
Heather Talbott
WB Bovine and Mouse 03/27/2017
View

Summary

ApplicationWestern Blot
Sample Testedbovine corpus luteum,bovine fat,bovine heart,bovine liver,Mouse heart,mouse liver
SpeciesBovine and Mouse
LotC-1

Comments

Comments20ug of sample/well
Block - 30min at room temperature 5% non-fat dry milk in 0.1% Tween 20 in TBS
Primary - 1:1000, 2h at room temperature 1% non-fat dry milk in 0.1% Tween 20 in TBS
Secondary - 1:10,000 anti-rabbit-HRP [Jackson 111-035-003 Lot 122107], 1:5,000 anti-biotin-HRP [Cell Signaling 7075P Lot 28] 2h at room temperature 1% non-fat dry milk in 0.1% Tween 20 in TBS
ECL - SuperSignal Fempto
UVP Biospectrum

Product General Protocols

Video Protocols

WB Video Protocol

FAQs for Perilipin-2/ADFP Antibody (NB110-40878). (Showing 1 - 2 of 2 FAQs).

  1. I would appreciate if you are able to provide the exact amino acid sequence of the immunogens. The customer would like to check the percentage alignment with the chicken protein to determine which of the antiodies woudl be better to try in this species.
    • I am unable to provide you with this information however I can tell you that there is a 71% homology between the chicken protein and human protein. Our lab only recommends use if the homology is 85% or above.
  2. May I ask what the recommended blocking buffer is for NB110-40878?
    • We validated this antibody using 5% milk plus 1% BSA in TBS-T as our blocking buffer for Western blot. This is our standard blocking buffer it isn't listed in the product specific protocol.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-40878AF350
Alexa Fluor 405 NB110-40878AF405
Alexa Fluor 488 NB110-40878AF488
Alexa Fluor 532 NB110-40878AF532
Alexa Fluor 594 NB110-40878AF594
Alexa Fluor 647 NB110-40878AF647
Alexa Fluor 700 NB110-40878AF700
Alexa Fluor 750 NB110-40878AF750
Biotin NB110-40878B
DyLight 350 NB110-40878UV
DyLight 405 NB110-40878V
DyLight 488 NB110-40878G
DyLight 550 NB110-40878R
DyLight 594 NB110-40878DL594
DyLight 650 NB110-40878C
DyLight 680 NB110-40878FR
DyLight 755 NB110-40878IR
FITC NB110-40878F
HRP NB110-40878H
Janelia Fluor 549 NB110-40878JF549
Janelia Fluor 646 NB110-40878JF646

Additional Perilipin-2/ADFP Products

Bioinformatics Tool for Perilipin-2/ADFP Antibody (NB110-40878)

Discover related pathways, diseases and genes to Perilipin-2/ADFP Antibody (NB110-40878). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Perilipin-2/ADFP Antibody (NB110-40878)

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Blogs on Perilipin-2/ADFP.

The affects of Perilipin 2 on diet and metabolism
Perilipin 2 belongs to the Perilipin family, which consists of proteins that coat intracellular lipid storage droplets. Perilipin 2 in particular is involved in lipid globule surface membrane composition, and has also been implicated in the develo...  Read full blog post.

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Recent Reviews

2.5
5
0
4
1
3
0
2
0
1
1

Verified Customer
09/15/2020
Application: IHC-Fr
Species: Pig

Heather Talbott
03/27/2017
Application: WB
Species: Bovine and Mouse

Bioinformatics

Gene Symbol PLIN2