TRAP1 Antibody (TRAP1-6)

Images

 
Western Blot: TRAP1 Antibody (TRAP1-6) [NB300-555] - Figure 2 illustrates IP of TRAP1 visualized by Coomassie Blue staining.
Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Analysis of TRAP1 in NCI-H460 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed ...read more
Immunohistochemistry-Paraffin: TRAP1 Antibody (TRAP1-6) [NB300-555] - Normal biopsies of deparaffinized Human liver tissue, dilution of 1:20.
Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Immunolocalization of TRAP1 in PC-3-M cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
TRAP1-6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TRAP1 Antibody (TRAP1-6) Summary

Immunogen
Purified recombinant TRAP1.
Localization
Mitochondrial
Specificity
Detects tumor necrosis factor receptor-associated protein (TRAP1) from human tissues.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TRAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:20
  • Immunohistochemistry-Paraffin 1:10 - 1:100
  • Immunoprecipitation 1:10 - 1:500
Application Notes
WB: Detects an approx. 75 kDa protein representing TRAP1.
Positive Control
TRAP1 Lysate (NBL1-17255)
Publications
Read Publications using
NB300-555 in the following applications:

Reactivity Notes

Mouse reported in literature (PMID: 21924824). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRAP1 Antibody (TRAP1-6)

  • HSP 75
  • HSP75TNFR-associated protein 1
  • HSP90Lheat shock protein 75 kDa, mitochondrial
  • TNF receptor-associated protein 1
  • TRAP-1
  • tumor necrosis factor type 1 receptor associated protein
  • Tumor necrosis factor type 1 receptor-associated protein

Background

The 90 kDa heat shock protein (hsp90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. Hsp90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of hsp90 is TRAP1. Like hsp90, TRAP1 is found to be associated with numerous proteins involved in diverse actions. Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein. Immunofluorescence staining of TRAP1 in PC-3-M cells with this antibody produces a pattern consistent with mitochondrial staining. Immunoprecipitation of TRAP1 using this antibody fails to co-precipitate p23, Hop, or CyP40 suggesting TRAP1's inability to associate with these co-chaperones.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-555
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for TRAP1 Antibody (NB300-555)(2)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRAP1 Antibody (NB300-555) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRAP1
Entrez