PDIA2 Antibody

Images

 
Western Blot: PDIA2 Antibody [NB100-1921] - Normal human brain.
Immunocytochemistry/ Immunofluorescence: PDIA2 Antibody [NB100-1921] - IF Confocal analysis of 3T3 cells using PDIA2 antibody (NB100-1921, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary ...read more
Immunohistochemistry-Paraffin: PDIA2 Antibody [NB100-1921] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human brain using 1:250 conc. of PDIA2 antibody on a Bond Rx autostainer (Leica Biosystems). The ...read more
Immunocytochemistry/ Immunofluorescence: PDIA2 Antibody [NB100-1921] - PDIA2 staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).
Immunoprecipitation: PDIA2 Antibody [NB100-1921] - Analysis of WDFY3 in E13.5 mouse embryos using anti-WDFY3 antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PDIA2 Antibody Summary

Immunogen
Purified full length PDIA2 isolated from rat liver.
Localization
Endoplasmic reticulum
Marker
Endoplasmic Reticulum Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDIA2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:5000-1:20000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1-3 ug
  • Western Blot 1:10000-1:20000
Application Notes
This PDIA2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen and paraffin embedded sections, Immunoprecipitation and Western Blot.
Control
Human Brain Frontal Lobe Membrane Tissue Lysate (Adult Membrane Normal)
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-1921 in the following applications:

Publications
Read Publications using
NB100-1921 in the following applications:

  • 2 publications
  • WB
    2 publications

Reactivity Notes

Human, rat, mouse, monkey, and bovine.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for PDIA2 Antibody

  • Pancreas-specific protein disulfide isomerase
  • pancreatic protein disulfide isomerase
  • PDA2
  • PDI
  • PDIp
  • PDIR
  • protein disulfide isomerase, pancreatic
  • protein disulfide isomerase-associated 2
  • protein disulfide-isomerase A2
  • Rho GDP dissociation inhibitor gamma

Background

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. The protein plays a role in the folding of nascent proteins in the endoplasmic reticulum by forming disulfide bonds through its thiol isomerase, oxidase, and reductase activity. The encoded protein also possesses estradiol-binding activity and can modulate intracellular estradiol levels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDIA2 Antibody (NB100-1921)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(2)
WB
(2)
All Applications
Filter By Species
Human
(3)
All Species

Reviews for PDIA2 Antibody (NB100-1921) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NB100-1921:
Filter by Applications
Western Blot
(1)
Immunocytochemistry
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PDIA2 NB100-1921
Enlarge
5
reviewed by:
Fabrice GANKAM
Western Blot Rat 03/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedRat brain
SpeciesRat
Immunocytochemistry PDIA2 NB100-1921
Enlarge
5
reviewed by:
Verified Customer
Immunocytochemistry Mouse 06/16/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDIA2 Antibody (NB100-1921). (Showing 1 - 1 of 1 FAQs).

  1. What permeabilization method was used to test this antibody?
    • For our protocol, our lab uses 0.1-0.5% Tween-20 in PBS or TBS for permeabilization.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Contact Information

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Product PDFs

Calculators

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Fabrice GANKAM
03/06/2015
Application: Western Blot
Species: Rat

Verified Customer
06/16/2014
Application: Immunocytochemistry
Species: Mouse

Bioinformatics

Gene Symbol PDIA2
Entrez