PDI Antibody

Images

 
Western Blot: PDI Antibody [NB100-1921] - Normal human brain.
Immunocytochemistry/ Immunofluorescence: PDI Antibody [NB100-1921] - IF Confocal analysis of 3T3 cells using PDI antibody (NB100-1921, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary ...read more
Immunohistochemistry-Paraffin: PDI Antibody [NB100-1921] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human brain using 1:250 conc. of PDI antibody on a Bond Rx autostainer (Leica Biosystems). The assay ...read more
Immunocytochemistry/ Immunofluorescence: PDI Antibody [NB100-1921] - PDI staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).
Immunoprecipitation: PDI Antibody [NB100-1921] - analysis of WDFY3 in E13.5 mouse embryos using anti-WDFY3 antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PDI Antibody Summary

Immunogen
Purified full length PDI isolated from rat liver.
Localization
Endoplasmic reticulum
Marker
Endoplasmic Reticulum Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDIA2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000-1:20000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1-3 ug
Application Notes
This PDI antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen and paraffin embedded sections, Immunoprecipitation and Western Blot.
Control
Human Brain Frontal Lobe Membrane Tissue Lysate (Adult Membrane Normal)
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-1921 in the following applications:

Publications
Read Publications using
NB100-1921 in the following applications:

  • 2 publications
  • WB
    2 publications

Reactivity Notes

Human, rat, mouse, monkey, and bovine.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for PDI Antibody

  • Pancreas-specific protein disulfide isomerase
  • pancreatic protein disulfide isomerase
  • PDA2
  • PDI
  • PDIp
  • PDIPEC 5.3.4.1
  • PDIR
  • protein disulfide isomerase A2
  • protein disulfide isomerase family A, member 2
  • protein disulfide isomerase, pancreatic
  • protein disulfide isomerase-associated 2
  • protein disulfide-isomerase A2
  • Rho GDP dissociation inhibitor gamma

Background

PDI (pancreas-specific protein disulfide isomerase; PDIA2) catalyzes the rearrangement of disulphide bonds (-S-S) in proteins and it was identified as a pancreas-specific member of PDI family which lead to its common name - PDIp. Highly expressed in pancreas with localization in ER lumen, PDI exist predominantly as monomer under reducing conditions, however, may form disulfide-linked homodimers which exhibits enhanced chaperone activity. PDI participates in a large chaperone multiprotein complex comprising of DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. PDI acts as an intracellular estrogen-binding protein, and implicates in modulating cellular levels or biological functions of estrogens in pancreas. Moreover, PDI has also been shown to play a role in the binding and regulation of intracellular 17beta-estradiol levels, thereby regulating estrogen biosynthesis. PDI also act as a chaperone which inhibits aggregation of misfolded proteins and has been proposed to play a key role in the production as well as secretion of digestive enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDI Antibody (NB100-1921)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(2)
WB
(2)
All Applications
Filter By Species
Human
(3)
All Species

Reviews for PDI Antibody (NB100-1921) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NB100-1921:
Filter by Applications
WB
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PDI NB100-1921
Enlarge
reviewed by:
Anonymous
WB Rat 03/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedRat brain
SpeciesRat

Blocking

Blocking DetailsMilk at 10%

Primary Anitbody

Dilution Ratio1/10000 ON in TBST0.05% at 4°

Secondary Antibody

Secondary DescriptionAnti Rabbit HRP 1/20000 from Pierce
Secondary Concentration1/20000

Details

Detection NotesECL 2 sec exposure tme with advanta sirius ECL reagent

Comments

CommentsExcellent antibody
Immunocytochemistry PDI NB100-1921
Enlarge
reviewed by:
Anony- mous
ICC Mouse 06/16/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesMouse
Special ApplicationsIF Confocal analysis of 3T3 cells using PDI antibody (NB100-1921, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDI Antibody (NB100-1921). (Showing 1 - 1 of 1 FAQs).

  1. What permeabilization method was used to test this antibody?
    • For our protocol, our lab uses 0.1-0.5% Tween-20 in PBS or TBS for permeabilization.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Blogs on PDI.

ERO1 Activity: A Potential Source of ER-Derived Oxidative Stress.
Disulfide bond formation is a pivotal step in the maturation and release of secretory proteins that are controlled by specific endoplasmic reticulum (ER) resident enzymes. An important element in this process is ERO (ER oxidoreduction), a glycosylated...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
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Anonymous
03/06/2015
Application: WB
Species: Rat

Anony- mous
06/16/2014
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol PDIA2
Entrez
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