Western Blot: PDI Antibody [NB100-1921] - Normal human brain.
Immunocytochemistry/ Immunofluorescence: PDI Antibody [NB100-1921] - IF Confocal analysis of 3T3 cells using PDI antibody (NB100-1921, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary ...read more
Immunohistochemistry-Paraffin: PDI Antibody [NB100-1921] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human brain using 1:250 conc. of PDI antibody on a Bond Rx autostainer (Leica Biosystems). The assay ...read more
Immunocytochemistry/ Immunofluorescence: PDI Antibody [NB100-1921] - PDI staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).
Immunoprecipitation: PDI Antibody [NB100-1921] - analysis of WDFY3 in E13.5 mouse embryos using anti-WDFY3 antibody. Image from verified customer review.
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Alternate Names for PDI Antibody
Pancreas-specific protein disulfide isomerase
pancreatic protein disulfide isomerase
protein disulfide isomerase A2
protein disulfide isomerase family A, member 2
protein disulfide isomerase, pancreatic
protein disulfide isomerase-associated 2
protein disulfide-isomerase A2
Rho GDP dissociation inhibitor gamma
PDI (pancreas-specific protein disulfide isomerase; PDIA2) catalyzes the rearrangement of disulphide bonds (-S-S) in proteins and it was identified as a pancreas-specific member of PDI family which lead to its common name - PDIp. Highly expressed in pancreas with localization in ER lumen, PDI exist predominantly as monomer under reducing conditions, however, may form disulfide-linked homodimers which exhibits enhanced chaperone activity. PDI participates in a large chaperone multiprotein complex comprising of DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. PDI acts as an intracellular estrogen-binding protein, and implicates in modulating cellular levels or biological functions of estrogens in pancreas. Moreover, PDI has also been shown to play a role in the binding and regulation of intracellular 17beta-estradiol levels, thereby regulating estrogen biosynthesis. PDI also act as a chaperone which inhibits aggregation of misfolded proteins and has been proposed to play a key role in the production as well as secretion of digestive enzymes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
IF Confocal analysis of 3T3 cells using PDI antibody (NB100-1921, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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