PDGFR alpha Antibody [Phycoerythrin]

NIH-3T3 mouse embryonic fibroblast cell line was stained with Goat Anti-Mouse PDGF Ra PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1062P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Phycoerythrin

PDGFR alpha Antibody [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha
  Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala)
  Accession # P26618
• Specificity: Detects mouse PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PDGF R alpha , rhPDGF R beta , and recombinant mouse PDGF R beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDGFRA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGFR alpha Antibody [Phycoerythrin]

• alpha-type platelet-derived growth factor receptor
• CD140 antigen-like family member A
• CD140a antigen
• CD140a
• EC 2.7.10
• EC 2.7.10.1
• MGC74795
• PDGF R alpha
• PDGFR alpha
• PDGFR2
• PDGFRA
• PDGFRA/BCR fusion
• PDGF-R-alpha
• platelet-derived growth factor receptor, alpha polypeptide
• rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
• RHEPDGFRA

Background

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB and -DD (1-4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha and beta receptors. Others have only the alpha receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages) (1, 2). Recombinant mouse and human soluble PDGF R beta bind PDGF with high affinity and are potent PDGF antagonists.

1. Betsholtz, C. et al. (2001) BioEssays 23:494.
2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGFR alpha Antibody (FAB1062P)(1)

Publication using FAB1062P

• Chaen, T;Kurosawa, T;Kishi, K;Kaji, N;Ikemoto-Uezumi, M;Uezumi, A;Hori, M; Transcriptome analysis of mesenchymal stromal cells of the large and small intestinal smooth muscle layers reveals a unique gastrontestinal stromal signature Biochemistry and biophysics reports 2023-07-01 [PMID: 37153863] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: PDGFRA
• Uniprot:
  • P26618()