Recombinant Human PDGF R beta Fc Chimera Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human PDGF R beta Fc Chimera Protein Summary

• Details of Functionality: Measured by its ability to inhibit the biological activity of PDGF-BB using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED 50 for this effect is 1-3 µg/mL in the presence of 50 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) .
• Source: Mouse myeloma cell line, NS0-derived human PDGF R beta protein

  Human PDGF R beta Leu33-Phe530 (Glu241Asp) Accession # P09619

  ENIEGRMD

  Human IgG 1 (Pro100-Lys330)

  6-His tag

  N-terminus

  C-terminus

• Accession #: P09619
• N-terminal Sequence: Leu33
• Protein/Peptide Type: Recombinant Proteins
• Gene: PDGFRB
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 84 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 94-124 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PDGF R beta Fc Chimera Protein

• beta-type platelet-derived growth factor receptor
• CD140 antigen-like family member B
• CD140b antigen
• CD140b
• EC 2.7.10
• EC 2.7.10.1
• JTK12
• PDGF R beta
• PDGFR
• PDGFR1
• PDGFRB
• PDGF-R-beta
• platelet-derived growth factor receptor, beta polypeptide

Background

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero-dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist.

1. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.

Publications for PDGF R beta (385-PR)(14)

Publications using 385-PR

• JA Contreras, K Fujisaki, N Ortega, G Barisano, A Sagare, I Pappas, H Chui, JM Ringman, EB Joe, B Zlokovic, AW Toga, J Pa Decreased functional connectivity is associated with increased levels of Cerebral Spinal Fluid soluble-PDGFRbeta, a marker of blood brain barrier breakdown, in older adults Research square, 2023-03-07;0(0):. 2023-03-07 [PMID: 36945439] (Reference Standard, Human)
• R Beckmann, K Jensen, S Fenn, J Speck, K Krause, A Meier, M Röth, S Fauser, R Kimbung, DT Logan, M Steegmaier, H Kettenberg DutaFabs are engineered therapeutic Fab fragments that can bind two targets simultaneously Nature Communications, 2021-01-29;12(1):708. 2021-01-29 [PMID: 33514724] (ELISA Capture, Human)
• J Park, KS Gill, AA Aghajani, JD Heredia, H Choi, A Oberstein, E Procko Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology PLoS Pathog., 2020-06-19;16(6):e1008647. 2020-06-19 [PMID: 32559251] (Bioassay, Human)
• Kuniyuki Nakamura, Tomoko Ikeuchi, Kazuki Nara, Craig S. Rhodes, Peipei Zhang, Yuta Chiba et al. Perlecan regulates pericyte dynamics in the maintenance and repair of the blood–brain barrier Journal of Cell Biology 2019-10-07 [PMID: 31541017] (Bioassay, Mouse)
• SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics Sci Rep, 2017-11-27;7(1):16439. 2017-11-27 [PMID: 29180757] (Surface Plasmon Resonance, Human)
• Z Tao, H Yang, Q Shi, Q Fan, L Wan, X Lu Targeted Delivery to Tumor-associated Pericytes via an Affibody with High Affinity for PDGFR? Enhances the in vivo Antitumor Effects of Human TRAIL Theranostics, 2017-06-01;7(8):2261-2276. 2017-06-01 [PMID: 28740549] (Bioassay)
• C Stegmann, D Hochdorfer, D Lieber, N Subramania, D Stöhr, K Laib Sampa, C Sinzger A derivative of platelet-derived growth factor receptor alpha binds to the trimer of human cytomegalovirus and inhibits entry into fibroblasts and endothelial cells PLoS Pathog., 2017-04-12;13(4):e1006273. 2017-04-12 [PMID: 28403220] (Bioassay, Human)
• Y Wu, A Prager, S Boos, M Resch, I Brizic, M Mach, S Wildner, L Scrivano, B Adler Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-? as a key for entry PLoS Pathog., 2017-04-12;13(4):e1006281. 2017-04-12 [PMID: 28403202] (Bioassay, Human)
• S Lykkemark, OA Mandrup, MB Jensen, J Just, P Kristensen A novel excision selection method for isolation of antibodies binding antigens expressed specifically by rare cells in tissue sections Nucleic Acids Res., 2017-06-20;0(0):. 2017-06-20 [PMID: 28369551] (ELISA (Standard), Human)
• Kun Ding Generation and characterization of ABBV642, a dual variable domain immunoglobulin molecule (DVD-Ig) that potently neutralizes VEGF and PDGF-BB and is designed for the treatment of exudative age-related macular degeneration MAbs, 2017-02-01;0(0):0. 2017-02-01 [PMID: 27929753] (Bioassay)
• Donica C, Cui Y, Shi S, Gutstein H Platelet-derived growth factor receptor-beta antagonism restores morphine analgesic potency against neuropathic pain. PLoS ONE, 2014-05-12;9(5):e97105. 2014-05-12 [PMID: 24820332] (In Vivo, Rat)
• Camorani S, Esposito C, Rienzo A, Catuogno S, Iaboni M, Condorelli G, de Franciscis V, Cerchia L Inhibition of receptor signaling and of glioblastoma-derived tumor growth by a novel PDGFRbeta aptamer. Mol Ther, 2014-01-02;22(4):828-41. 2014-01-02 [PMID: 24566984] (Bioassay, Human)
• Vascular endothelial growth factor signals through platelet-derived growth factor receptor beta in meningiomas in vitro. Br. J. Cancer, 2012-10-09;107(10):1702-13. 2012-10-09 [PMID: 23047550] (Bioassay)
• Lacoste E, Martineau I, Gagnon G Platelet concentrates: effects of calcium and thrombin on endothelial cell proliferation and growth factor release. J. Periodontol., 2003-10-01;74(10):1498-507. 2003-10-01 [PMID: 14653397] (ELISA (Capture), Human)

Review for PDGF R beta (385-PR) (1)

Average Rating: 4

(Based on 1 review)

reviewed by: Steven Moore In vivo study 10/13/2020

Summary

• Application: In vivo study
• Lot: Recombinant Human PDGF R beta Fc Chimera Protein AMO0916081
• Comments: publishable, would use again

Comments

• Comments: Recombinant wave recorded in the intact cellular structure

Bioinformatics

• Gene Symbol: PDGFRB
• Uniprot:
  • P09619()