PDGF R beta [p Tyr751] Antibody (1210B) [Unconjugated]

Western blot shows lysates of CCD-1070Sk human foreskin fibroblast cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 20 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse Phospho-PDGF R beta (Y751) Monoclonal Antibody (Catalog # MAB9027) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PDGF R beta at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PDGF R beta phosphorylated at Y751 was detected in immersion fixed BJ human skin fibroblast cell line stimulated (top panel), and unstimulated (bottom panel), with Recombinant Human PDGF-BB (Catalog # 220-BB) using Rabbit Anti-Human/Mouse Phospho-PDGF R beta (Y751) Monoclonal Antibody (Catalog # MAB9027) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF‑BB (Catalog # 220-BB) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for PDGF R beta at approximately 240 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse Phospho-PDGF R beta (Y751) Monoclonal Antibody (Catalog # MAB9027). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF
• Clone: 1210B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PDGF R beta [p Tyr751] Antibody (1210B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Phosphopeptide containing the human PDGF R beta (Y751) site
  Accession # P09619
• Modification: p Tyr751
• Specificity: Detects human and mouse PDGF R beta when phosphorylated at Y751 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PDGFRB
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-25 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGF R beta [p Tyr751] Antibody (1210B) [Unconjugated]

• beta-type platelet-derived growth factor receptor
• CD140 antigen-like family member B
• CD140b antigen
• CD140b
• EC 2.7.10
• EC 2.7.10.1
• JTK12
• PDGF R beta
• PDGFR
• PDGFR1
• PDGFRB
• PDGF-R-beta
• platelet-derived growth factor receptor, beta polypeptide

Background

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero-dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist.

1. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGF R beta Antibody (MAB9027)(2)

Publications using MAB9027

• Subramanian Dharmarajan, Casandra Carrillo, Zhonghua Qi, Jonathan M. Wilson, Anthony J. Baucum, Christine M. Sorenson et al. Retinal inflammation in murine models of type 1 and type 2 diabetes with diabetic retinopathy Diabetologia 2023-09-05 [PMID: 37670018]
• CS Farrar, DC Hocking Assembly of fibronectin fibrils selectively attenuates platelet-derived growth factor-induced intracellular calcium release in fibroblasts J. Biol. Chem., 2018-10-15;0(0):. 2018-10-15 [PMID: 30323067] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PDGFRB
• Uniprot:
  • P09619()