PDGF-BB Antibody [Unconjugated]

Recombinant Human PDGF‑BB (Catalog # 220-BB) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human PDGF‑BB (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-BB Polyclonal Antibody (Catalog # AB-220-NA). The ND50 is typically 0.25‑1.5 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PDGF-BB Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PDGF‑BB
  Ser82-Thr190
  Accession # P01127
• Specificity: Detects human PDGF-BB in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant human (rh) PDGF-AB is observed, approximately 50% cross-reactivity with recombinant rat PDGF-B and natural porcine PDGF is observed, and less than 10% cross-reactivity with rhPDGF-A is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDGFB
• Purity Statement: Protein A or G purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.25-1.5 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGF-BB Antibody [Unconjugated]

• c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV
• PDGFBB
• PDGF-BB

Background

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta . Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGF-BB Antibody (AB-220-NA)(5)

Publications using AB-220-NA

• SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis International Journal of Molecular Sciences, 2022-01-14;23(2):. 2022-01-14 [PMID: 35055054] (Neutralization, Human)
• YY Liang, XB Deng, XT Lin, LL Jiang, XT Huang, ZW Mo, YW Yuan, MT Teh RASSF1A inhibits PDGFB-driven malignant phenotypes of nasopharyngeal carcinoma cells in a YAP1-dependent manner Cell Death Dis, 2020-10-14;11(10):855. 2020-10-14 [PMID: 33057010] (Neutralization, Human)
• Sohinee Bhattacharyya, Chet Oon, Aayush Kothari, Wesley Horton, Jason Link, Rosalie C. Sears et al. Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer Journal of Experimental Medicine 2020-08-03 [PMID: 32434218] (Neutralization, Human)
• Y Zou, S Xu, Y Xiao, Q Qiu, M Shi, J Wang, L Liang, Z Zhan, X Yang, N Olsen, SG Zheng, H Xu Long noncoding RNA LERFS negatively regulates rheumatoid synovial aggression and proliferation J. Clin. Invest., 2018-09-10;0(0):. 2018-09-10 [PMID: 30198906] (Neutralization, Human)
• Chanakira A, Kir D, Barke R, Santilli S, Ramakrishnan S, Roy S Hypoxia Differentially Regulates Arterial and Venous Smooth Muscle Cell Migration. PLoS ONE, 2015-09-18;10(9):e0138587. 2015-09-18 [PMID: 26381529] (Neutralization, Human)

Bioinformatics

• Gene Symbol: PDGFB
• Uniprot:
  • P01127()