PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free

Western Blot: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - Loading: 15 ug of HeLa WT cell lysates or PD-L2 KO celllysates. Antibodies: PD-L2, 4063 (4 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000dilution

Western Blot: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - Loading: 15 ug of lysates per lane. Antibodies: PD-L2 (4 ug/mL), competitor antibody (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/Immunofluorescence: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - analysis of 4% paraformaldehydefixed mouse brain cells labeling PD-L2 with this antibody at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (red).

Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - Staining of mouse brain tissue with antibody at 2.5 ug/ml.

Western Blot: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - Analysis of PD-L2 in Raji cell lysate with PD-L2 antibody at 0.5 and 1 ug/mL.

Western Blot: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - Loading: 15 ug of HeLa WT cell lysates or PD-L2 KO cell lysates. Antibodies: PD-L2, NBP1-76770 (4 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry-Paraffin: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - Mouse brain tissue.

Immunocytochemistry/ Immunofluorescence: PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free [NBP1-76770] - Validation of PD-L2/B7-DC/PDCD1LG2 in Mouse Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling PD-L2/B7-DC/PDCD1LG2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 16 amino acids near the center of human PD-L2/B7-DC/PDCD1LG2. The immunogen is located within amino acids 140-190 of PD-L2/B7-DC/PDCD1LG2. Amino Acid Sequence: SVPANTSHSRTPEGLY
• Specificity: Human PD-L2/B7-DC/PDCD1LG2 has 3 isoforms, including isoform 1 (273aa, 31kD), isoform 2 (183aa, 21kD) and isoform 3 (182aa, 21kD). Mouse PD-L2/B7-DC/PDCD1LG2 has one isoform (247aa, 28kD) and Rat PD-L2/B7-DC/PDCD1LG2 also has one isoform (268aa, 30kD). NBP1-76770 can detect human isoform and mouse isoform.
• Predicted Species: Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDCD1LG2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin
  • Western Blot 0.5-4 ug/mL
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  PD-L2/B7-DC/PDCD1LG2 Peptide (NBP1-76770PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for PD-L2/B7-DC/PDCD1LG2 Antibody - BSA Free

• B7-DC
• bA574F11.2
• Btdc
• Butyrophilin B7-DC
• Butyrophilin-like Protein
• CD273 antigen
• CD273
• CD273PD-1 ligand 2
• MGC142240
• PD-1-ligand 2
• PDCD1L2MGC142238
• PDCD1LG2
• PDL2
• PD-L2
• PDL2B7DC
• PD-L2PDCD1 ligand 2
• programmed cell death 1 ligand 2
• Programmed death ligand 2

Background

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PDL-2 can act to stimulate an immunogenic response through and alternative receptor from PD-1. At least three isoforms of PDL-2 are known to exist; this antibody is specific to the largest isoform. PDL-2 antibody has no cross-reactivity to PDL-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PD-L2/B7-DC/PDCD1LG2 Antibody (NBP1-76770)(1)

Publication using NBP1-76770

• Guo H, Zhang C, Shen YK et Al. PD-L2 mediates tobacco smoking-induced recruitment of regulatory T cells via the RGMB/NF?B/CCL20 cascade Cell Biol Toxicol 2024-07-23 [PMID: 39042313]

Review for PD-L2/B7-DC/PDCD1LG2 Antibody (NBP1-76770) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 06/21/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Lung tissue
• Species: Human

Bioinformatics

• Gene Symbol: PDCD1LG2
• Uniprot:
  • NP_079515()