PD-L2/B7-DC/PDCD1LG2 Antibodies

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="PD-L2/B7-DC was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human PD-L2/B7-DC Monoclonal Antibody (Catalog # MAB1224) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="PD-L2 was detected in perfusion fixed frozen sections of mouse thymus using 15 µg/mL Goat Anti-Mouse PD-L2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1022) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF017). Specific bands were detected for PD-L2/B7-DC at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). Specific bands were detected for PD-L2/B7-DC at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human lung tissue and HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD-L2/B7-DC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1224) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PD-L2/B7-DC at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0113). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0113). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either mouse PD-L2/B7-DC (filled histogram) or irrelevant transfectants (open histogram) was stained with Rat Anti-Mouse PD-L2/B7-DC Monoclonal Antibody (Catalog # MAB1022), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

AFR1086) binds to HEK293 cells transfected with Human PD-L2 and eGFP. (A) Binding is completely blocked by 25 µg/mL of Mouse Anti-Human PD‑L2/B7‑DC Monoclonal Antibody (Catalog # MAB11351) but not by (B) Mouse IgG₁ Isotype Control (Catalog # MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> AFR1086) binds to HEK293 cells transfected with Human PD-L2 and eGFP. (A) Binding is completely blocked by 25 µg/mL of Mouse Anti-Human PD‑L2/B7‑DC Monoclonal Antibody (Catalog # MAB11351) but not by (B) Mouse IgG₁ Isotype Control (Catalog # MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol." alt="In a functional flow cytometry test, Recombinant Human PD-1 Fc Chimera Alexa Fluor® 647 Protein (AFR1086) binds to HEK293 cells transfected with Human PD-L2 and eGFP. (A) Binding is completely blocked by 25 µg/mL of Mouse Anti-Human PD‑L2/B7‑DC Monoclonal Antibody (Catalog # MAB11351) but not by (B) Mouse IgG₁ Isotype Control (Catalog # MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either (A) human PD-L2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human PD-L2 Monoclonal Antibody (Catalog # MAB12241) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

MAB12243) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human PD-L2/B7-DC Monoclonal Antibody (Catalog # MAB12242) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB12243) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human PD-L2/B7-DC Monoclonal Antibody (Catalog # MAB12242) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human PD-L2/B7-DC protein was serially diluted 2-fold and captured by Mouse Anti-Human PD-L2/B7-DC Monoclonal Antibody (Catalog # MAB12243) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human PD-L2/B7-DC Monoclonal Antibody (Catalog # MAB12242) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

DY990). Mouse Anti-Human PD-L2/B7-DC Monoclonal Antibody (Catalog # MAB12242) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Mouse Anti-Human PD-L2/B7-DC Monoclonal Antibody (Catalog # MAB12242) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human PD-L2/B7-DC protein was serially diluted 2-fold and captured by Mouse Anti-Human PD-L2/B7-DC Monoclonal Antibody (Catalog # MAB12243) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human PD-L2/B7-DC Monoclonal Antibody (Catalog # MAB12242) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />