Genetic Strategies: Knockdown Validated: PD-L1 Antibody [NBP1-76769] - Validation with PD-L1 siRNA Knockdown. HeLa cells were transfected with control siRNAs (lane 1) or PD-L1 siRNAs (lane 2). Loading: 10 ug of ...read more
Independent Antibodies: Western Blot: PD-L1 Antibody [NBP1-76769] - Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 15 ug of lysates per lane.Antibodies: NBP1-76769 (2 ug/mL), PD-L1 ...read more
Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - CD274 (PD-L1) expression in the HNSCC patients from the TCGA database, HNSCC tissue samples and the HNSCC cells. Immunostaining of PD-L1 obtained from HNSCC ...read more
Western Blot: PD-L1 Antibody [NBP1-76769] - PD-L1 expression was evaluated in gastric cancer cells. Levels of PD-L1 protein were assessed in normal human gastric epithelial cells and 8 gastric cancer cell lines by ...read more
Biological Strategies: Western Blot: PD-L1 Antibody [NBP1-76769] - Upregulated expression of PD-L1 in cisplatin-resistant cells through IL-6/STAT3. Image collected and cropped by Cite Ab from the following ...read more
Western Blot: PD-L1 Antibody [NBP1-76769] - CD274 (PD-L1) expression in the HNSCC patients from the TCGA databas HNSCC cells. CD274 expression with survival and its relation with therapy in the HNSCC patients from the ...read more
Flow Cytometry: PD-L1 Antibody [NBP1-76769] - B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - Analysis of A-20 cells using B7-H1/PD-L1/CD274 antibody at 0.5 ug/mL. Green: Isotype control. Red : B7-H1/PD-L1/CD274 antibody.
Western Blot: PD-L1 Antibody [NBP1-76769] - Expression of CD274 in Human melanoma SK-MEL-28 cell line upon treatment with 200 mM ethanol. Dilution: 1:1,000 in PBS with 5% BSA. Secondary Ab: anti-Rabbit IgG 1:5,000. ...read more
Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - Human tonsil tissue with PD-L1 antibody at 5 ug/mL.
Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - Staining of human heart tissue with antibody at 2.5 ug/mL.
Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - PD-L1/B7-H1 Antibody [NBP1-76769] - PD-L1 in rat heart tissue with at 5 ug/mL.
Immunofluorescence: PD-L1 Antibody [NBP1-76769] - Human Heart cells with PD-L1 antibody at 20 ug/mL.
Use in ICC/IF reported in scientific literature (PMID:33220359) Use in IHC-Frozen was reported in scientific literature (PMID: 28402953). Use in immunoprecipitation reported in scientific literature (PMID: 28978117).
37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Peptide affinity purified
Alternate Names for PD-L1 Antibody
PD-L1B7 homolog 1
PDL1PDCD1 ligand 1
programmed cell death 1 ligand 1
Programmed death ligand 1
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. PDL-1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PDL-1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. At least two isoforms of PDL-1 are known to exist; this antibody is specific to the larger isoform. PDL-1 antibody has no cross-reactivity to PDL-2.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Paraffin-embedded tissue blocks of 2 different samples were cut into 2 μm sections. Automated deparaffinization and rehydration was performed by immersing the slides in Xylene, in graded alcohol series and finally in water as indicated. 0.3% hydrogen peroxide was used to block endogenous peroxidase activity for 30 minutes. Antigen retrieval was performed for each sample both in citrate 10 mM (pH 6.0) buffer and in Tris-EDTA 10 mM (pH 9.0) buffer at 98°C for 30 minutes. Primary antibody was used both at final dilution of 5 ug/mL and 10 ug/mL in 1.5% horse serum in PBS, and sections were incubated for 2 hours at room temperature. Incubation with biotinylated secondary antibody and streptavidin/HRP complex were performed using the VECTASTAIN® Universal Quick Kit Peroxidase, R.T.U. (Vector Laboratories) following manufacturer’s instructions. DAB was added to tissue sections until brown color developed (3 minutes), then slides were moved to water for 5 minutes and counterstained in hematoxylin. Finally, slides were dehydrated and mounted. *Low Rating Note: Novus Innovators Program - new species or application used on a primary antibody.
HCT-116 colon cancer cell line w/wo drug treatment
I tested protein lording amount, 25, 30, and 50 ug to optimize the PD-L1 expression. Blocking buffer was Li-COR synthetic protein blocking buffer for 1 hr. The dilution of Primary antibody(PD-L1, NBP1-76769) were 1:500, 1:1000 and 1:2000 that I tested. Secondary antibody, Li-COR, IRdye 800CW goat anti-rabbit, 1:5000 dilution for 45 min.
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