PD-L1 Antibody

Images

 
Genetic Strategies: Knockdown Validated: PD-L1 Antibody [NBP1-76769] - Validation with PD-L1 siRNA Knockdown. HeLa cells were transfected with control siRNAs (lane 1) or PD-L1 siRNAs (lane 2). Loading: 10 ug of ...read more
Independent Antibodies: Western Blot: PD-L1 Antibody [NBP1-76769] - Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 15 ug of lysates per lane.Antibodies: NBP1-76769 (2 ug/mL), PD-L1 ...read more
Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - CD274 (PD-L1) expression in the HNSCC patients from the TCGA database, HNSCC tissue samples and the HNSCC cells. Immunostaining of PD-L1 obtained from HNSCC ...read more
Western Blot: PD-L1 Antibody [NBP1-76769] - PD-L1 expression was evaluated in gastric cancer cells. Levels of PD-L1 protein were assessed in normal human gastric epithelial cells and 8 gastric cancer cell lines by ...read more
Biological Strategies: Western Blot: PD-L1 Antibody [NBP1-76769] - Upregulated expression of PD-L1 in cisplatin-resistant cells through IL-6/STAT3. Image collected and cropped by Cite Ab from the following ...read more
Western Blot: PD-L1 Antibody [NBP1-76769] - CD274 (PD-L1) expression in the HNSCC patients from the TCGA databas HNSCC cells. CD274 expression with survival and its relation with therapy in the HNSCC patients from the ...read more
Flow Cytometry: PD-L1 Antibody [NBP1-76769] - B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - Analysis of A-20 cells using B7-H1/PD-L1/CD274 antibody at 0.5 ug/mL. Green: Isotype control. Red : B7-H1/PD-L1/CD274 antibody.
Western Blot: PD-L1 Antibody [NBP1-76769] - Expression of CD274 in Human melanoma SK-MEL-28 cell line upon treatment with 200 mM ethanol. Dilution: 1:1,000 in PBS with 5% BSA. Secondary Ab: anti-Rabbit IgG 1:5,000. ...read more
Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - Human tonsil tissue with PD-L1 antibody at 5 ug/mL.
Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - Staining of human heart tissue with antibody at 2.5 ug/mL.
Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - PD-L1/B7-H1 Antibody [NBP1-76769] - PD-L1 in rat heart tissue with at 5 ug/mL.
Immunofluorescence: PD-L1 Antibody [NBP1-76769] - Human Heart cells with PD-L1 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ICC/IF, IHC-P (-), KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PD-L1 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide from near the center of human PD-L1. The immunogen is located within amino acids 60-110 of PD-L1.
Specificity
PD-L1 antibody has no cross-reactivity to PD-L2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD274
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 0.5 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Immunofluorescence 20 ug/ml
  • Knockdown Validated
Application Notes
Use in ICC/IF reported in scientific literature (PMID:33220359) Use in IHC-Frozen was reported in scientific literature (PMID: 28402953). Use in immunoprecipitation reported in scientific literature (PMID: 28978117).
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
PD-L1 Knockout HeLa Cell Lysate
PD-L1 Knockout A549 Cell Lysate
Reviewed Applications
Read 5 Reviews rated 3.2
using
NBP1-76769 in the following applications:

Publications
Read Publications using
NBP1-76769 in the following applications:

  • 2 publications
  • IHC
    4 publications
  • 1 publication
  • 2 publications
  • IP
    1 publication
  • KD
    1 publication
  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for PD-L1 Antibody

  • Avelumab
  • B7-H
  • B7H1
  • B7-H1
  • B7H1PDCD1L1
  • CD274 antigenMGC142294
  • CD274 molecule
  • CD274
  • PDCD1L1
  • PDCD1LG1
  • PDCD1LG1MGC142296
  • PDL1
  • PD-L1
  • PD-L1B7 homolog 1
  • PDL1PDCD1 ligand 1
  • programmed cell death 1 ligand 1
  • Programmed death ligand 1

Background

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. PDL-1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PDL-1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. At least two isoforms of PDL-1 are known to exist; this antibody is specific to the larger isoform. PDL-1 antibody has no cross-reactivity to PDL-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PD-L1 Antibody (NBP1-76769)(20)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: ICC/IF, IHC, IHC-F, IHC-P, IP, KD, WB.


Filter By Application
ICC/IF
(2)
IHC
(4)
IHC-F
(1)
IHC-P
(2)
IP
(1)
KD
(1)
WB
(6)
All Applications
Filter By Species
Human
(11)
Mouse
(6)
Rat
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NBP1-76769 Applications Species
Mondal T, Shivange GN, Tihagam RG et al. Unexpected PD-L1 immune evasion mechanism in TNBC, ovarian, and other solid tumors by DR5 agonist antibodies EMBO molecular medicine Feb 15 2021 [PMID: 33587338]
Holokai L, Chakrabarti J, Lundy J et al. Murine- and Human-Derived Autologous Organoid/Immune Cell Co-Cultures as Pre-Clinical Models of Pancreatic Ductal Adenocarcinoma Cancers Dec 17 2020 [PMID: 33348809] (ICC/IF, Human) ICC/IF Human
Yang A, Li MY, Zhang ZH et al. Erianin regulates programmed cell death ligand 1 expression and enhances cytotoxic T lymphocyte activity J Ethnopharmacol Nov 18 2020 [PMID: 33220359] (IHC, ICC/IF) IHC, ICC/IF
Zhou J, Wu Z, Hu J et al. High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations EMBO J Nov 20 2020 [PMID: 33219024]
Tsutaho A, Hashimoto A, Hashimoto S et al. High expression of AMAP1, an ARF6 effector, is associated with elevated levels of PD-L1 and fibrosis of pancreatic cancer Cell Commun. Signal Jun 24 2020 [PMID: 32580737] (IHC, Human) IHC Human
Li Z, Zhang C, Du JX et al. Adipocytes promote tumor progression and induce PD-L1 expression via TNF-alpha/IL-6 signaling Cancer Cell Int. May 20 2020 [PMID: 32477009] (WB, Human, Mouse) WB Human, Mouse
Liu C, Ma Z, Cai Z et Al. Identification of primordial germ cell-like cells as liver metastasis initiating cells in mouse tumour models Cell Discov Mar 24 2020 [PMID: 32218989] (IHC, Mouse) IHC Mouse
Wang, X;Wu, WKK;Gao, J;Li, Z;Dong, B;Lin, X;Li, Y;Li, Y;Gong, J;Qi, C;Peng, Z;Yu, J;Shen, L; Autophagy inhibition enhances PD-L1 expression in gastric cancer J. Exp. Clin. Cancer Res. Mar 29 2019 [PMID: 30925913] (IHC-P, Human) IHC-P Human
Chakrabarti J, Holokai L, Syu L et al. Hedgehog signaling induces PD-L1 expression and tumor cell proliferation in gastric cancer. Oncotarget Dec 21 2018 [PMID: 30647844] (WB, Mouse) WB Mouse
Kozak K, Szumera-Cieckiewicz A. 1441PPROGRAMMED DEATH LIGAND-1 (PD-L1) EXPRESSION AND PROGNOSTIC VALUE IN SYNOVIAL SARCOMA. Annals of Oncology. 2014
Show All 20 Publications.

Reviews for PD-L1 Antibody (NBP1-76769) (5) 3.25

Average Rating: 3.2
(Based on 5 reviews)
We have 5 reviews tested in 4 species: Human, Mouse, Canine, Mouse and Human.

Reviews using NBP1-76769:
Filter by Applications
IHC-P
(2)
ICC
(1)
WB
(2)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Canine
(1)
Mouse and Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin PD-L1 NBP1-76769
Enlarge
1
reviewed by:
Valeria Martini
IHC-P Canine 08/27/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedFFPE
SpeciesCanine
LotB

Comments

CommentsParaffin-embedded tissue blocks of 2 different samples were cut into 2 μm sections.
Automated deparaffinization and rehydration was performed by immersing the slides in Xylene, in graded alcohol series and finally in water as indicated.
0.3% hydrogen peroxide was used to block endogenous peroxidase activity for 30 minutes.
Antigen retrieval was performed for each sample both in citrate 10 mM (pH 6.0) buffer and in Tris-EDTA 10 mM (pH 9.0) buffer at 98°C for 30 minutes.
Primary antibody was used both at final dilution of 5 ug/mL and 10 ug/mL in 1.5% horse serum in PBS, and sections were incubated for 2 hours at room temperature.
Incubation with biotinylated secondary antibody and streptavidin/HRP complex were performed using the VECTASTAIN® Universal Quick Kit Peroxidase, R.T.U. (Vector Laboratories) following manufacturer’s instructions.
DAB was added to tissue sections until brown color developed (3 minutes), then slides were moved to water for 5 minutes and counterstained in hematoxylin.
Finally, slides were dehydrated and mounted.
*Low Rating Note: Novus Innovators Program - new species or application used on a primary antibody.
Immunocytochemistry PD-L1 NBP1-76769
Enlarge
3
reviewed by:
Anonymous
ICC Mouse 05/12/2018
View

Summary

ApplicationImmunocytochemistry
Sample Testedpancreas cancer cells
SpeciesMouse
LotNBP1-76769

Comments

Commentsdilution 1:100 overnight incubation @ 4 C
Western Blot PD-L1 NBP1-76769
Enlarge
5
reviewed by:
Anonymous
WB Human 03/02/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman melanoma
SpeciesHuman
Lot7315-1303
  4
reviewed by:
Anonymous
IHC-P Mouse and Human 01/18/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedtransgenic mouse pancreas,Pancreatic cancer tissue
SpeciesMouse and Human
Lot8293-1504-2
Western Blot PD-L1 NBP1-76769
Enlarge
3
reviewed by:
Anonymous
WB Human 11/09/2016
View

Summary

ApplicationWestern Blot
Sample TestedHCT-116 colon cancer cell line w/wo drug treatment
SpeciesHuman

Comments

CommentsI tested protein lording amount, 25, 30, and 50 ug to optimize the PD-L1 expression.
Blocking buffer was Li-COR synthetic protein blocking buffer for 1 hr.
The dilution of Primary antibody(PD-L1, NBP1-76769) were 1:500, 1:1000 and 1:2000 that I tested.
Secondary antibody, Li-COR, IRdye 800CW goat anti-rabbit, 1:5000 dilution for 45 min.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PD-L1 Antibody (NBP1-76769). (Showing 1 - 1 of 1 FAQs).

  1. What can I use as positive controls for B7-H1 (NBP1-76769) other than the myocardial tissue?
    • For CD274 (B7-H1), this protein is highly expressed in the heart, skeletal muscle, placenta and lung ( uniprot site). It can also be found at lower levels in other tissues as well: protein atlas site.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-76769AF350
Alexa Fluor 405 NBP1-76769AF405
Alexa Fluor 488 NBP1-76769AF488
Alexa Fluor 532 NBP1-76769AF532
Alexa Fluor 594 NBP1-76769AF594
Alexa Fluor 647 NBP1-76769AF647
Alexa Fluor 700 NBP1-76769AF700
Alexa Fluor 750 NBP1-76769AF750
Allophycocyanin NBP1-76769APC
Biotin NBP1-76769B
DyLight 350 NBP1-76769UV
DyLight 405 NBP1-76769V
DyLight 488 NBP1-76769G
DyLight 550 NBP1-76769R
DyLight 594 NBP1-76769DL594
DyLight 650 NBP1-76769C
DyLight 680 NBP1-76769FR
DyLight 755 NBP1-76769IR
FITC NBP1-76769F
HRP NBP1-76769H
Janelia Fluor 549 NBP1-76769JF549
Janelia Fluor 646 NBP1-76769JF646
PE NBP1-76769PE
PerCP NBP1-76769PCP

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Recent Reviews

3.2
5
1
4
1
3
2
2
0
1
1

Valeria Martini
08/27/2020
Application: IHC-P
Species: Canine

Anonymous
05/12/2018
Application: ICC
Species: Mouse

Anonymous
03/02/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol CD274
Uniprot