G-102-C)." class="big_lightbox"> G-102-C)." class="big_thumb" />

8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.70 uM." class="big_lightbox"> 8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.70 uM." class="big_thumb" />

9047-PD) was measured at a concentration range between 1.53 nM and 1.56 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.539 uM. " class="big_lightbox"> 9047-PD) was measured at a concentration range between 1.53 nM and 1.56 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.539 uM. " class="big_thumb" />

1086-PD) with an ED₅₀ of 0.05-0.3 µg/mL." class="big_lightbox"> 1086-PD) with an ED₅₀ of 0.05-0.3 µg/mL." alt="When Recombinant Human PD-L1 / B7-H1 is immobilized at 0.025 µg/mL, it binds to Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) with an ED₅₀ of 0.05-0.3 µg/mL." class="big_thumb" />

1086-PD) is immobilized at 2 µg/mL, Biotinylated Recombinant Human PD-L1 His-tag Avi-tag protein binds with an ED₅₀ of 2-12 µg/mL." class="big_lightbox"> 1086-PD) is immobilized at 2 µg/mL, Biotinylated Recombinant Human PD-L1 His-tag Avi-tag protein binds with an ED₅₀ of 2-12 µg/mL." alt="When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is immobilized at 2 µg/mL, Biotinylated Recombinant Human PD-L1 His-tag Avi-tag protein binds with an ED₅₀ of 2-12 µg/mL." class="big_thumb" />

F0050), which does not stain the cells in the absence of recombinant protein (B)." class="big_lightbox"> F0050), which does not stain the cells in the absence of recombinant protein (B)." class="big_thumb" />

8986-PD) was measured at a concentration range between 3.2 nM and 13.2 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.122 µM." class="big_lightbox"> 8986-PD) was measured at a concentration range between 3.2 nM and 13.2 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.122 µM." class="big_thumb" />

1021-PD) with an ED₅₀ of 8-40 ng/mL." class="big_lightbox"> 1021-PD) with an ED₅₀ of 8-40 ng/mL." alt="When Recombinant Mouse PD-L1/B7-H1 is immobilize at 25 ng/mL, it binds Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) with an ED₅₀ of 8-40 ng/mL." class="big_thumb" />

1021-PD) with an ED₅₀ of 15.0‑150 ng/mL." class="big_lightbox"> 1021-PD) with an ED₅₀ of 15.0‑150 ng/mL." alt="Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Mouse PD-L1/B7-H1 His-tag Avi-tag Protein (Catalog # AVI9048) binds to Recombinant Mouse PD-1 Fc Chimera Protein (1021-PD) with an ED₅₀ of 15.0‑150 ng/mL." class="big_thumb" />

1086-PD) that produces 50% of the optimal binding response is found to be approximately 0.5-3.0 µg/mL." class="big_lightbox"> 1086-PD) that produces 50% of the optimal binding response is found to be approximately 0.5-3.0 µg/mL." alt="When Recombinant Porcine PD-L1/B7-H1 His-tag Protein (Catalog # 10361-B7) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) that produces 50% of the optimal binding response is found to be approximately 0.5-3.0 µg/mL." class="big_thumb" />

1021-PD) is immobilized at 2.5 μg/mL (100 μL/well), Biotinylated Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag Protein (Catalog # AVI1019) binds with an ED₅₀ of 0.0600-0.600 μg/mL." class="big_lightbox"> 1021-PD) is immobilized at 2.5 μg/mL (100 μL/well), Biotinylated Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag Protein (Catalog # AVI1019) binds with an ED₅₀ of 0.0600-0.600 μg/mL." alt="Measured by its binding ability in a functional ELISA. When Recombinant Mouse PD-1 Fc Chimera Protein (1021-PD) is immobilized at 2.5 μg/mL (100 μL/well), Biotinylated Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag Protein (Catalog # AVI1019) binds with an ED₅₀ of 0.0600-0.600 μg/mL." class="big_thumb" />

1086-PD) is coated at 1 µg/mL, Biotinylated Recombinant Human PD-L1/B7-H1 Fc Chimera Avi-tag (Catalog # AVI156) binds with an ED₅₀ of 8-48 ng/mL." class="big_lightbox"> 1086-PD) is coated at 1 µg/mL, Biotinylated Recombinant Human PD-L1/B7-H1 Fc Chimera Avi-tag (Catalog # AVI156) binds with an ED₅₀ of 8-48 ng/mL." alt="When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is coated at 1 µg/mL, Biotinylated Recombinant Human PD-L1/B7-H1 Fc Chimera Avi-tag (Catalog # AVI156) binds with an ED₅₀ of 8-48 ng/mL." class="big_thumb" />

10553-PD) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Canine PD-L1/B7-H1 Fc Chimera (Catalog # 10746-B7) binds with an ED₅₀ of 0.30-4.50 µg/mL." class="big_lightbox"> 10553-PD) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Canine PD-L1/B7-H1 Fc Chimera (Catalog # 10746-B7) binds with an ED₅₀ of 0.30-4.50 µg/mL." alt="When Recombinant Canine PD-1 Fc Chimera (10553-PD) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Canine PD-L1/B7-H1 Fc Chimera (Catalog # 10746-B7) binds with an ED₅₀ of 0.30-4.50 µg/mL." class="big_thumb" />