PD-1 Antibody - BSA Free

Western Blot: PD-1 Antibody [NBP1-77277] - THP-1 cell lysate with PD-1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.

Immunocytochemistry/ Immunofluorescence: PD-1 Antibody - BSA Free [NBP1-77277] - Immunocytochemistry of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 10 ug/ml.

Immunocytochemistry/ Immunofluorescence: PD-1 Antibody - BSA Free [NBP1-77277] - Immunofluorescence of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 20 ug/ml.Green: PD-1 Antibody Blue: DAPI staining

Immunohistochemistry: PD-1 Antibody - BSA Free [NBP1-77277] - Immunohistochemistry of PD-1 in human brain tissue with PD-1 antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PD-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PD-1 Antibody - BSA Free (NBP1-77277) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the center of human PD-1. The immunogen is located within amino acids 120 - 170 of PD-1. Amino Acid Squence: LRAELRVTERRAEVPT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDCD1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  PD-1 Peptide (NBP1-77277PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for PD-1 Antibody - BSA Free

• CD279 antigen
• CD279
• hPD-1
• PD1
• PD-1
• PD1hPD-l
• PDCD1
• programmed cell death 1
• programmed cell death protein 1
• Protein PD-1
• SLEB2

Background

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for PD-1 Antibody (NBP1-77277). (Showing 1 - 1 of 1 FAQ).

1. Is this anitbody IgG? Which subtype does it belong to? 
   • It  is IgG and is affinity chromatography purified via peptide column.

Bioinformatics

• Gene Symbol: PDCD1
• Uniprot:
  • Q15116()