PCNA Antibody

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Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500
Immunocytochemistry/ Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PCNA protein stained by PCNA antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale ...read more
Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Paraffin-embedded mouse liver. PCNA antibody dilution: 1:500.
Western Blot: PCNA Antibody [NBP1-32075] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody diluted at a dilution of 1:2500.
Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug NIH-3T3 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500
Immunocytochemistry/ Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PCNA protein stained by PCNA antibody diluted at 1:500. Red: Phalloidin, a ...read more
Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis.
Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Paraffin-embedded human colon. PCNA antibody diluted at 1:500.
Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Paraffin-embedded human cervical carcinoma. PCNA antibody diluted at 1:500.
Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Paraffin-embedded rat hind brain. PCNA antibody dilution: 1:500.
Immunoprecipitation: PCNA Antibody [NBP1-32075] - Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 ug of PCBP2 antibody. Western blot analysis was performed using PCBP2 antibody. EasyBlot ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Mk, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.14 mg/ml

Order Details

PCNA Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.
Localization
Nucleus
Marker
Proliferation Marker
Predicted Species
Porcine (99%), Monkey (100%), Zebrafish (92%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PCNA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-32075 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Rhesus Monkey (100%), Xenopus laevis (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.14 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PCNA Antibody

  • cyclin
  • DNA polymerase delta auxiliary protein
  • MGC8367
  • proliferating cell nuclear antigen

Background

PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PCNA Antibody (NBP1-32075)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PCNA Antibody (NBP1-32075) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for PCNA Antibody (NBP1-32075)

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Blogs on PCNA.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Caspase 3 - a Reliable Marker for Index of Apoptosis Induction
Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and...  Read full blog post.

PCNA is a Universal Marker of Proliferating Cells
Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. PCNA was first shown to be involved in DNA replication. However PCNA functions are associated with other vital...  Read full blog post.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.

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Bioinformatics

Gene Symbol PCNA