PCNA [p Tyr211] Antibody

Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast tissue. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used at at dilution of 1:250 and incubated for 18 hours at room temperature. Detection: DAB

Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used a dilution of 1:250 and incubated for 18 hours at room temeprature. Detection: DAB

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.05 mg/ml

PCNA [p Tyr211] Antibody Summary

• Description: Novus Biologicals Rabbit PCNA [p Tyr211] Antibody (NB100-59861) is a polyclonal antibody validated for use in IHC. Anti-PCNA Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human proliferating cell nuclear antigen (GeneID 5111) around tyrosine 211 according to the numbering given inentry NP_002583.1.
• Modification: p Tyr211
• Marker: Proliferation Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCNA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
• Application Notes: Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Extended incubation with primary antibody (16-24 hours) at room temperature is recommended. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.
• Control:

  PCNA Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.05 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PCNA [p Tyr211] Antibody

• cyclin
• DNA polymerase delta auxiliary protein
• MGC8367
• PCNA
• proliferating cell nuclear antigen

Background

PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PCNA Antibody (NB100-59861)(1)

Publication using NB100-59861

• Zhao H, Lo YH, Ma L et al. Targeting tyrosine phosphorylation of PCNA inhibits prostate cancer growth. Mol Cancer Ther. 2011-01-01 [PMID: 21220489]
  
  Details:
  Prostate Cancer (IMH-303). IHC (paraffin): The tissuse microarray was stained with phospho-Y211 PCNA antibody, Fig 1

Bioinformatics

• Gene Symbol: PCNA
• Entrez:
  • 5111(Human)
• Uniprot:
  • P12004(Human)