PCNA Antibody [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

Order Details

PCNA Antibody [DyLight 650] Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residues 75-125 of human proliferating cell nuclear antigen using the SwissProt entry P12004 (GeneID 5111).
Marker
Proliferation Marker
Predicted Species
Rat (100%), Chicken (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PCNA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PCNA Antibody [DyLight 650]

  • cyclin
  • DNA polymerase delta auxiliary protein
  • MGC8367
  • proliferating cell nuclear antigen

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PCNA Antibody (NB100-456C) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-456AF350
Alexa Fluor 405 NB100-456AF405
Alexa Fluor 488 NB100-456AF488
Alexa Fluor 532 NB100-456AF532
Alexa Fluor 594 NB100-456AF594
Alexa Fluor 647 NB100-456AF647
Alexa Fluor 700 NB100-456AF700
Alexa Fluor 750 NB100-456AF750
Allophycocyanin NB100-456APC
Allophycocyanin/Cy7 NB100-456APCCY7
DyLight 350 NB100-456UV
DyLight 405 NB100-456V
DyLight 488 NB100-456G
DyLight 550 NB100-456R
DyLight 594 NB100-456DL594
DyLight 650 NB100-456C
DyLight 680 NB100-456FR
DyLight 755 NB100-456IR
FITC NB100-456F
HRP NB100-456H
Janelia Fluor 549 NB100-456JF549
Janelia Fluor 646 NB100-456JF646
PE NB100-456PE
PE/Atto594 NB100-456PEATT594
PE/Cy5.5 NB100-456PECY55
PE/Cy7 NB100-456PECY7
PerCP NB100-456PCP

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Bioinformatics Tool for PCNA Antibody (NB100-456C)

Discover related pathways, diseases and genes to PCNA Antibody (NB100-456C). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Pathways for PCNA Antibody (NB100-456C)

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Research Areas for PCNA Antibody (NB100-456C)

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Blogs on PCNA.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Caspase 3 - a Reliable Marker for Index of Apoptosis Induction
Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and...  Read full blog post.

PCNA is a Universal Marker of Proliferating Cells
Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. PCNA was first shown to be involved in DNA replication. However PCNA functions are associated with other vital...  Read full blog post.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.

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Bioinformatics

Gene Symbol PCNA
Entrez
Uniprot