PBR Antibody

Western Blot: PBR Antibody [NBP1-51934] - Staining of Mouse Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence

Immunohistochemistry-Paraffin: PBR Antibody [NBP1-51934] - Negative Control showing staining of paraffin embedded Mouse Kidney, with no primary antibody.

Flow Cytometry: PBR Antibody [NBP1-51934] - Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: PBR Antibody [NBP1-51934] - Staining of paraffin embedded Mouse Kidney. Antibody at 10 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining (1:500).

• Reactivity: Mu, Hu, Rt
• Applications: WB, ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PBR Antibody Summary

• Description: Novus Biologicals Goat PBR Antibody (NBP1-51934) is a polyclonal antibody validated for use in IHC, WB, ELISA and Flow. Anti-PBR Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RDNSGRRGGSRLPE corresponding to C-Terminus according to Uniprot Mouse NP_033905.3.
• Epitope: C-RDNSGRRGGSRLPE
• Predicted Species: Human (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TSPO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunohistochemistry 5 - 10 ug/mL
  • Immunohistochemistry-Paraffin 5 - 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.01 - 0.03 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PBR Antibody

• benzodiazepine peripheral binding site
• BZRPDBI
• IBP
• MBRPBS
• mDRC
• Mitochondrial benzodiazepine receptor
• PBR
• PBRBPBS
• PBRS
• peripheral benzodiazepine receptor
• Peripheral-type benzodiazepine receptor
• pk18
• PKBSbenzodiazapine receptor (peripheral)
• PTBR
• translocator protein (18kDa)
• translocator protein
• TSPO

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PBR Antibody (NBP1-51934)(1)

Publication using NBP1-51934

• Weisinger G, Kelly-Hershkovitz E, Veenman L et al. Peripheral benzodiazepine receptor antisense knockout increases tumorigenicity of MA-10 Leydig cells in vivo and in vitro. Biochemistry 2004-09-28 [PMID: 15379570]

Bioinformatics

• Gene Symbol: TSPO
• Entrez:
  • 12257(Mouse)
  • 24230(Rat)
• Uniprot:
  • NP_033905.3(Mouse)