PBEF/Visfatin/NAMPT Antibody

Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-19467] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Visfatin antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: NAMPT Antibody [NBP2-19467] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NBP2-19467] - Paraffin-embedded mouse liver. Visfatin antibody diluted at 1:500.

Western Blot: NAMPT Antibody [NBP2-19467] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:3000.

Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NBP2-19467] - Paraffin-embedded rat testis. Visfatin antibody diluted at 1:500.

Product Discontinued

PBEF/Visfatin/NAMPT Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Visfatin. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Porcine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NAMPT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (87%), Chicken (84%), Bovine (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for PBEF/Visfatin/NAMPT Antibody

• EC 2.4.2.12
• MGC117256
• NAmPRTase
• NAMPT
• nicotinamide phosphoribosyltransferase
• NMPRTase
• PBEF
• PBEF1
• PBEF1110035O14Rik
• PBEF1DKFZp666B131
• Pre-B cell-enhancing factor
• pre-B-cell colony enhancing factor 1
• Pre-B-cell colony-enhancing factor 1
• VF
• Visfatin

Background

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NAMPT
• Uniprot:
  • P43490()