Pax6 Antibody [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

Pax6 Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human Pax6
  Met1-Arg272
  Accession # P26367
• Specificity: Detects human Pax6 in direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Intracellular Staining by Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax6 Antibody [Alexa Fluor® 532]

• keratitis)
• MGC17209
• Oculorhombin
• paired box 6
• paired box protein Pax-6
• Pax6

Background

Pax6 (paired box 6; also Oculorhombin) is a 48‑50 kDa member of the paired homeobox family of transcription factors.  It is expressed in developing optic vesicle, olfactory dopaminergic neurons, and pancreatic endocrine cells.  Pax6 is a transactivating protein that interacts with MAF, CDX2 and SOX2.  Human Pax6 is 422 amino acids (aa) in length.  It contains an N-terminal paired box DNA-binding domain (aa 4‑130), a Gly-rich central region (aa 131‑209), a homeodomain (aa 213‑269) and a C-terminal Pro/Ser/Thr-rich regulatory domain (aa 279‑422).  Phosphorylation of the C‑terminal domain at Thr281/304/373 promotes Pax6 activity.  Multiple splice forms of Pax6 exist. There are alternative start sites at Met137 and a position 34 aa upstream of the standard site. There is also a deletion of aa 22‑26 and 37‑39, plus a 14 aa insertion after Gln47 that generates a C‑terminal DNA binding site.  Human and mouse Pax6 are absolutely identical in aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Pax6 Antibody (AF8150X). (Showing 1 - 1 of 1 FAQ).

1. What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?
   • By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.