PARP2 Antibody

Immunohistochemistry-Paraffin: PARP2 Antibody [NBP2-47337] - Staining in human testis and pancreas tissues using NBP2-47337 antibody. Corresponding PARP2 RNA-seq data are presented for the same tissues.

Western Blot: PARP2 Antibody [NBP2-47337] - Analysis in human cell line U-2 OS and human cell line SK-MEL-30.

Immunocytochemistry/Immunofluorescence: PARP2 Antibody [NBP2-47337] - Staining of human cell line MCF7 shows localization to nucleus. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: PARP2 Antibody [NBP2-47337] - Staining of human pancreas shows no positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: PARP2 Antibody [NBP2-47337] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PARP2 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: ARALNESKRVNNGNTAPEDSSPAKKTRRCQRQESKKMPVAGGKANKDRTEDKQDESVKALLLKGKAPVDPECTAKVGKAHVYCEGNDVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWM
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PARP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PARP2 Protein (NBP2-47337PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PARP2 Antibody

• ADP-ribosyltransferase (NAD+; poly(ADP-ribose) polymerase)-like 2
• ADPRT2
• ADPRT-2
• ADPRT2pADPRT-2
• ADPRTL2
• ADPRTL2EC 2.4.2.30
• ADPRTL3
• ARTD2
• EC 2.4.2.30
• hPARP-2
• NAD(+) ADP-ribosyltransferase 2
• pADPRT-2
• PARP2
• PARP-2
• poly (ADP-ribose) polymerase 2
• poly (ADP-ribose) polymerase family, member 2
• poly (ADP-ribosyl) transferase-like 2
• poly [ADP-ribose] polymerase 2
• poly(ADP-ribose) synthetase
• Poly[ADP-ribose] synthase 2
• poly[ADP-ribose] synthetase 2

Background

PARP2 encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PARP2