PARP2 Antibody - BSA Free

Western Blot: PARP2 Antibody [NBP2-47337] - Analysis in human cell line U-2 OS and human cell line SK-MEL-30.

Immunocytochemistry/Immunofluorescence: PARP2 Antibody [NBP2-47337] - Staining of human cell line MCF7 shows localization to nucleus. Antibody staining is shown in green.

Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Staining of human cerebellum shows strong nuclear positivity in cells in granular and molecular layers, as well as in Purkinje cells.

Staining of human cervix shows moderate to strong nuclear positivity in squamous epithelial cells.

Staining of human duodenum shows strong nuclear positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PARP2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: ARALNESKRVNNGNTAPEDSSPAKKTRRCQRQESKKMPVAGGKANKDRTEDKQDESVKALLLKGKAPVDPECTAKVGKAHVYCEGNDVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWM
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PARP2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PARP2 Protein (NBP2-47337PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PARP2 Antibody - BSA Free

• ADP-ribosyltransferase (NAD+; poly(ADP-ribose) polymerase)-like 2
• ADPRT2
• ADPRT-2
• ADPRT2pADPRT-2
• ADPRTL2
• ADPRTL2EC 2.4.2.30
• ADPRTL3
• ARTD2
• EC 2.4.2.30
• hPARP-2
• NAD(+) ADP-ribosyltransferase 2
• pADPRT-2
• PARP2
• PARP-2
• poly (ADP-ribose) polymerase 2
• poly (ADP-ribose) polymerase family, member 2
• poly (ADP-ribosyl) transferase-like 2
• poly [ADP-ribose] polymerase 2
• poly(ADP-ribose) synthetase
• Poly[ADP-ribose] synthase 2
• poly[ADP-ribose] synthetase 2

Background

PARP2 encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PARP2 Antibody (NBP2-47337)(2)

Publications using NBP2-47337

• C Morris, S Durand, P Jalinot Decreased expression of the translation factor eIF3e induces senescence in breast cancer cells via suppression of PARP1 and activation of mTORC1 Oncotarget, 2021-03-30;12(7):649-664. 2021-03-30 [PMID: 33868586]
• Katja Eloranta, Stefano Cairo, Emmi Liljeström, Tea Soini, Antti Kyrönlahti, Jean-Gabriel Judde, David B. Wilson, Markku Heikinheimo, Marjut Pihlajoki Chloroquine Triggers Cell Death and Inhibits PARPs in Cell Models of Aggressive Hepatoblastoma Frontiers in Oncology 2020-07-17 [PMID: 32766148]

Bioinformatics

• Gene Symbol: PARP2