Nanos2 Antibody

Images

 
Western Blot: Nanos2 Antibody [NBP1-76917] - Rat brain tissue lysate with Nanos2 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: Nanos2 Antibody [NBP1-76917] - Immunofluorescence of Nanos2 in Human Testis cells with Nanos2 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: Nanos2 Antibody [NBP1-76917] - Human brain tissue cells with Nanos2 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Nanos2 Antibody Summary

Immunogen
Antibody was raised against a 13 amino acid synthetic peptide near the amino terminus of the human Nanos2. The immunogen is located within the first 50 amino acids of Nanos2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NANOS2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/mL
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin 2.5 ug/mL
Control Peptide
Nanos2 Peptide (NBP1-76917PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nanos2 Antibody

  • nanos homolog 2 (Drosophila)
  • nanos homolog 2
  • Nanos2
  • NOS2NOS-2

Background

Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects. This Nanos2 antibody will not cross-react with either Nanos 1 or Nanos3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NANOS2
Entrez
Uniprot