| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27335 | |||
| BSA Free | NB100-56599SS | |||
| Azide and BSA Free | NBP2-27335SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13732-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF-600-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8095-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44461 | |||
| BSA Free | NBP2-36747-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44462 | |||
| BSA Free | NBP2-36748-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36748 | |||
| BSA Free | NBP2-36748-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26439-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26438-50ul | |||
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Western-Blot-NB100-56599-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Immunocytochemistry-Immunofluorescence-NB100-56599-img0005.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. Image from verified customer review.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Western-Blot-NB100-56599-img0003.jpg)
![Western Blot: PARP Antibody [NBP2-13732] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PARP-Antibody-Western-Blot-NBP2-13732-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PARP Antibody [NBP2-13732] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PARP Antibody [NBP2-13732] - Staining of human cell line HEK 293 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13732-img0007.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0014.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human lymph node shows strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0010.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human pancreas shows strong nuclear positivity.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0011.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human parathyroid gland shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0012.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0013.jpg)
![Western Blot: PARP Antibody (CL2220) [NBP2-36747] - analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PARP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Western-Blot-NBP2-36747-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PARP Antibody (CL2220) [NBP2-36747] - analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PARP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining in U251 cell line showing cell cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0012.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunohistochemistry-Paraffin-NBP2-36747-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining in HeLa cell line showing cell cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining of human cell line A431 showing cell cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining in MCF7 cell line showing clear cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining in U2OS cell line with Anti-PARP1 monoclonal antibody, showing cell cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0011.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human tonsil shows strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunohistochemistry-Paraffin-NBP2-36747-img0015.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunohistochemistry-Paraffin-NBP2-36747-img0016.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunohistochemistry-Paraffin-NBP2-36747-img0017.jpg)
![Immunocytochemistry/ Immunofluorescence: PARP Antibody (2G8N7) [PARP] - Immunofluorescence analysis of paraffin-embedded human lung using PARP Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15802_rabbit-parp-mab-2g8n7-52202517284973.jpg "Immunocytochemistry/ Immunofluorescence: PARP Antibody (2G8N7) [PARP] - Immunofluorescence analysis of paraffin-embedded human lung using PARP Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Rat brain using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15802_rabbit-parp-mab-2g8n7-52202517162320.jpg)
![Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15802_rabbit-parp-mab-2g8n7-52202517213585.jpg)
![Immunocytochemistry/ Immunofluorescence: PARP Antibody (2G8N7) [PARP] - Immunofluorescence analysis of HeLa cells using PARP Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15802_rabbit-parp-mab-2g8n7-52202517162397.jpg)
![Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15802_rabbit-parp-mab-2g8n7-5220251722393.jpg)
![Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15802_rabbit-parp-mab-2g8n7-52202517192939.jpg)
![Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Human spleen using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15802_rabbit-parp-mab-2g8n7-52202517284986.jpg)
![Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15802_rabbit-parp-mab-2g8n7-52202517235759.jpg)
![Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15802_rabbit-parp-mab-2g8n7-52202517192959.jpg)
![Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Human colon using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15802_rabbit-parp-mab-2g8n7-52202517223955.jpg)
![Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15802_rabbit-parp-mab-2g8n7-52202517192934.jpg)
![Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Rat heart using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15802_rabbit-parp-mab-2g8n7-52202517172873.jpg)
![Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Mouse colon using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15802_rabbit-parp-mab-2g8n7-52202517251990.jpg)
![Immunohistochemistry: PARP Antibody (2G8N7) [PARP] - Immunohistochemistry analysis of paraffin-embedded Rat liver using PARP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15802_rabbit-parp-mab-2g8n7-5220251721357.jpg)
![Western Blot: PARP Antibody (CL2209) [NBP2-36748] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PARP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2209-Western-Blot-NBP2-36748-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PARP Antibody (CL2209) [NBP2-36748] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PARP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: PARP Antibody (CL2209) [NBP2-36748] - Staining of human liver shows nuclear immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2209-Immunohistochemistry-Paraffin-NBP2-36748-img0012.jpg)
![Western Blot: PARP Antibody (CL2209) [NBP2-36748] - Lane 1: Marker [kDa]. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/PARP-Antibody-CL2209-Western-Blot-NBP2-36748-img0006.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2209) [NBP2-36748] - Staining of human skin shows strong nuclear immunoreactivity in both epidermis and dermis.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2209-Immunohistochemistry-Paraffin-NBP2-36748-img0008.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2209) [NBP2-36748] - Staining of human cerebellum shows strong nuclear positivity in granular and molecular layer cells, as well as in Purkinje cells.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2209-Immunohistochemistry-Paraffin-NBP2-36748-img0009.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2209) [NBP2-36748] - Staining of human placenta shows strong nuclear immunoreactivity in trophoblast.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2209-Immunohistochemistry-Paraffin-NBP2-36748-img0010.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2209) [NBP2-36748] - Staining of human kidney shows nuclear positivity in both tubules and glomeruli.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2209-Immunohistochemistry-Paraffin-NBP2-36748-img0011.jpg)
![Lane 1: Marker [kDa]Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp3-44462_mouse-parp-mab-cl2209-azide-and-bsa-free-28220251217311.jpg)
![Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-262202415475644.jpg "Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-2722024850467.jpg)
![Western Blot: PARP Antibody (8C7) [NBP3-26439] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: PARP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 KDa Observed band size: 114 kDa](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-282202411485824.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (8C7) [NBP3-26439] - Staining of Hela Cells with PARP Antibody (8C7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-28220241323611.jpg)
![Flow Cytometry: PARP Antibody (8C7) [NBP3-26439] - Overlay histogram showing Jurkat cells stained with PARP Antibody (8C7) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-28220241431270.jpg)
![Western Blot: PARP Antibody [NBP3-09070] - Western blot analysis of PARP1 on Daudi and 293 cell lysates using anti- PARP1 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/PARP-Antibody-Western-Blot-NBP3-09070-img0001.jpg "Western Blot: PARP Antibody [NBP3-09070] - Western blot analysis of PARP1 on Daudi and 293 cell lysates using anti- PARP1 antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: PARP Antibody [NBP3-09070] - ICC staining PARP1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-09070-img0003.jpg)
![Flow Cytometry: PARP Antibody [NBP3-09070] - Flow cytometric analysis of K562 cells with PARP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PARP-Antibody-Flow-Cytometry-NBP3-09070-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody [NBP3-09070] - ICC staining PARP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-09070-img0002.jpg)
![Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-262202415355711.jpg "Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-272202485637.jpg)
![Western Blot: PARP Antibody (7C11) [NBP3-26438] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: PARP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 kDa Observed band size: 114 kDa](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-282202411472110.jpg)
![Western Blot: PARP Antibody (4M9X4) - Cleaved [NBP3-15813] - Western blot analysis of extracts of various cell lines, using PARP antibody (NBP3-15813) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PARP-Antibody-4M9X4-Cleaved-Western-Blot-NBP3-15813-img0001.jpg "Western Blot: PARP Antibody (4M9X4) - Cleaved [NBP3-15813] - Western blot analysis of extracts of various cell lines, using PARP antibody (NBP3-15813) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunoprecipitation: PARP Antibody (4M9X4) - Cleaved [PARP] - Immunoprecipitation analysis of 600 ug extracts of Jurkat cells using 3 ug PARP antibody . Western blot was performed from the immunoprecipitate using PARP antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-15813_rabbit-parp-mab-4m9x4-cleaved-52202517184530.jpg)