AIF Antibody [Unconjugated]

Images

 
Western blot shows lysates of MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, CTLL‑2 mouse cytotoxic T cell line, DA3 mouse myeloma cell line, and PC‑12 rat adrenal ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for AIF at approximately 69 kDa (as ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and AIF knockout HEK293T cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit ...read more
Apoptosis Inducing Factor (AIF) was detected in immersion fixed MCF-7 human breast cancer cell line using Rabbit Anti-Human/Mouse/Rat AIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1457) at 10 µg/mL for ...read more
AIF was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse/Rat AIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1457) at 15 µg/mL for 3 hours at ...read more
AIF was detected in immersion fixed staurosporine-stimulated Jurkat human acute T cell leukemia cell line using Rabbit Anti-Human/Mouse/Rat AIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1457) at 10 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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AIF Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human AIF
Glu121-Asp613
Accession # O95831
Specificity
Detects human, mouse, and rat mitochondria-processed AIF.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AIFM1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Simple Western 1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Knockout Validated
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF1457 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AIF Antibody [Unconjugated]

  • AIF
  • AIFapoptosis-inducing factor 1, mitochondrial
  • AIFM1
  • apoptosis-inducing factor, mitochondrion-associated, 1
  • COXPD6
  • MGC111425
  • PDCD8
  • PDCD8striatal apoptosis-inducing factor
  • programmed cell death 8 (apoptosis-inducing factor)
  • Programmed cell death protein 8

Background

Apoptosis-inducing factor (AIF, also known as programmed cell death protein 8) is a 58 kDa member of the FAD-dependent oxidoreductase family of molecules. It is ubiquitously expressed and found in the mitochondrial intermembrane space. AIF likely acts as a mitochondrial antioxidant providing protection via NADH oxidase activity. Upon release from the mitochondria, AIF passes into the nucleus where it initiates apoptosis. Human AIF precursor is 67 kDa in size and 613 amino acids (aa) in length and contains a cleavable N-terminal 102 aa mitochondrial localization sequence, followed by a spacer region (aa 103‑129) and an oxidoreductase domain (aa 130‑613) that possesses an NLS (aa 446‑451). Over aa 121‑613, human AIF shares 95% aa identity with mouse AIF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AIF Antibody (AF1457)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, Western Blot.


Filter By Application
ICC
(1)
IHC
(1)
IHC-Fr
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(3)
Mouse
(3)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF1457 Applications Species
H Nakajima, M Itakura, T Kubo, A Kaneshige, N Harada, T Izawa, YT Azuma, M Kuwamura, R Yamaji, T Takeuchi Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Aggregation Causes Mitochondrial Dysfunction during Oxidative Stress-induced Cell Death J. Biol. Chem, 2017;292(11):4727-4742. 2017 [PMID: 28167533] (Western Blot, Human) Western Blot Human
Delivery of a survivin promoter-driven antisense survivin-expressing plasmid DNA as a cancer therapeutic: a proof-of-concept study Onco Targets Ther, 2016;9(0):2601-13. 2016 [PMID: 27217778] (Western Blot, Human) Western Blot Human
L Admoni-Eli, I Nakdimon, A Shteinfer, T Prezma, T Arif, N Arbel, A Melkov, O Zelichov, I Levi, V Shoshan-Ba Novel Biomarker Proteins in Chronic Lymphocytic Leukemia: Impact on Diagnosis, Prognosis and Treatment PLoS ONE, 2016;11(4):e0148500. 2016 [PMID: 27078856] (Western Blot, Human) Western Blot Human
Hwang J, Hsu C, Yu W, Liu T, Yang W Diet-induced obesity exacerbates auditory degeneration via hypoxia, inflammation, and apoptosis signaling pathways in CD/1 mice. PLoS ONE, 2013;8(4):e60730. 2013 [PMID: 23637762] (IHC, Mouse) IHC Mouse
Hisatomi T, Nakao S, Murakami Y, Noda K, Nakazawa T, Notomi S, Connolly E, She H, Almulki L, Ito Y, Vavvas DG, Ishibashi T, Miller JW The regulatory roles of apoptosis-inducing factor in the formation and regression processes of ocular neovascularization. Am. J. Pathol., 2012;181(1):53-61. 2012 [PMID: 22613025] (IHC-Fr, Mouse) IHC-Fr Mouse
Notomi S, Hisatomi T, Kanemaru T, Takeda A, Ikeda Y, Enaida H, Kroemer G, Ishibashi T Critical involvement of extracellular ATP acting on P2RX7 purinergic receptors in photoreceptor cell death. Am. J. Pathol., 2011;179(6):2798-809. 2011 [PMID: 21983632] (ICC, Mouse) ICC Mouse

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 IC1457G
Biotin BAF1457

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Bioinformatics

Gene Symbol AIFM1
Uniprot