PARL Antibody - Azide and BSA Free

• Reactivity: Hu, Mu, Rt, Ch, Pm
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PARL Antibody - Azide and BSA Free Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: PARL Antibody was prepared from whole rabbit serum produced by repeated immunization from a synthetic peptide corresponding to the C terminus region of human PARL. (Uniprot: Q9H300)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PARL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:60000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Western Blot 1 ug/ml
• Application Notes: This product is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~42.2 kDa corresponding to the appropriate cell lysate or extract.

Reactivity Notes

Cross-reactivity with PARL (presenilin associated, rhomboid-like) from other sources has not been determined

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for PARL Antibody - Azide and BSA Free

• EC 3.4.21.105
• mitochondrial
• PARL
• presenilin associated, rhomboid-like
• PRO2207
• PSARL1
• RHBDS1
• rhomboid 7 homolog 1

Background

presenilin associated, rhomboid-like. This gene encodes a mitochondrial integral membrane protein. Following proteolytic processing of this protein, a small peptide (P-beta) is formed and translocated to the nucleus. This gene may be involved in signal transduction via regulated intramembrane proteolysis of membrane-tethered precursor proteins. Variation in this gene has been associated with increased risk for type 2 diabetes. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PARL Antibody (NBP1-44262)(2)

Publications using NBP1-44262

• Jeyaraju DV, Xu L, Letellier MC, Bandaru S, Zunino R, Berg EA, McBride HM, Pellegrini L. Phosphorylation and cleavage of presenilin-associated rhomboid-like protein (PARL) promotes changes in mitochondrial morphology. Proc Natl Acad Sci U S A;103(49):18562-7. 2006-12-05 [PMID: 17116872]
• Cipolat S, Rudka T, Hartmann D, Costa V, Serneels L, Craessaerts K, Metzger K, Frezza C, Annaert W, D'Adamio L, Derks C, Dejaegere T, Pellegrini L, D'Hooge R, Scorrano L, De Strooper B. Mitochondrial rhomboid PARL regulates cytochrome c release during apoptosis via OPA1-dependent cristae remodeling. Cell;126(1):27-9. 2006-07-14 [PMID: 16839884]

Bioinformatics

• Gene Symbol: PARL
• Uniprot:
  • Q9H300(Human)