SNX16 Antibody

Images

 
Western Blot: SNX16 Antibody [NB100-1211] - HEK293 overexpressing SNX16 and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: SNX16 Antibody [NB100-1211] - Staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: SNX16 Antibody [NB100-1211] - (0.05ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: SNX16 Antibody [NB100-1211] - Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: SNX16 Antibody [NB100-1211] - Staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SNX16 Antibody Summary

Immunogen
Peptide with sequence ATPYVPVPMPIGN-C corresponding to N-Terminus according to NP_690049.1, NP_690050.1.
Specificity
This antibody is expected to recognise both isoform a (NP_071416.2; NP_690049.1) and isoform b (NP_690050.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SNX16
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.05 - 0.2 ug/ml
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 38 kDa band observed in Daudi lysates (calculated MW of 39.2 kDa band according to NP_071416.2). In transfected HEK293 transiently expressing SNX16 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293.
IHC: Paraffin embedded Human Placenta, Tonsil and Breast.
Positive Control
SNX16 Lysate (NBL1-16307)
Publications
Read Publication using NB100-1211.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SNX16 Antibody

  • DKFZp666H147
  • sorting nexin 16
  • sorting nexin-16

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. The function of this protein has not been determined. This gene results in three transcript variants encoding two distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SNX16 Antibody (NB100-1211)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SNX16
Entrez
Uniprot