SNX16 Antibody

Western Blot: SNX16 Antibody [NB100-1211] - HEK293 overexpressing SNX16 and probed with (mock transfection in first lane).

Immunohistochemistry-Paraffin: SNX16 Antibody [NB100-1211] - Staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: SNX16 Antibody [NB100-1211] - (0.05ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: SNX16 Antibody [NB100-1211] - Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: SNX16 Antibody [NB100-1211] - Staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SNX16 Antibody Summary

• Immunogen: Peptide with sequence ATPYVPVPMPIGN-C corresponding to N-Terminus according to NP_690049.1, NP_690050.1.
• Specificity: This antibody is expected to recognise both isoform a (NP_071416.2; NP_690049.1) and isoform b (NP_690050.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SNX16
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.05 - 0.2 ug/ml
• Application Notes: WB: Approx. 38 kDa band observed in Daudi lysates (calculated MW of 39.2 kDa band according to NP_071416.2). In transfected HEK293 transiently expressing SNX16 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293.
  IHC: Paraffin embedded Human Placenta, Tonsil and Breast.
• Control:

  SNX16 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SNX16 Antibody

• DKFZp666H147
• sorting nexin 16
• sorting nexin-16

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. The function of this protein has not been determined. This gene results in three transcript variants encoding two distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SNX16 Antibody (NB100-1211)(1)

Publication using NB100-1211

• Teasdale RD, Loci D, Houghton F et al. A large family of endosome-localized proteins related to sorting nexin 1. Biochem J 2001-08-15 [PMID: 11485546]

Bioinformatics

• Gene Symbol: SNX16
• Entrez:
  • 64089(Human)
  • 74718(Mouse)
  • 64088(Rat)
• Uniprot:
  • NP_071416(Human)