Park7/DJ-1 Antibody (4H4) - BSA Free

Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Western blot shows lysates of 293T human embryonic kidney parental cell line and PARK7 knockout (KO) 293T cell line. PVDF membrane was probed with Mouse Anti-Human PARK7 Monoclonal Antibody (Catalog # NBP1-92715) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for PARK7 at approximately 23 kDa (as indicated) in the parental 293T cell line, but is not detectable in the knockout 293T cell line. This experiment was conducted under reducing conditions.

Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Analysis of whole brain and cell lysates using mouse mAb against DJ1/Park7, NBP1-92715, dilution 1:5,000 in green. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HeLa, [6] HEK293, and [7] C6 cells. The DJ1 antibody detects protein with apparent molecular weight of 21kDa but only in human cell lines, since it does not recognize the rat or mouse DJ1 protein. The blot was simultaneously probed with chicken pAb to vimentin, dilution 1;5,000 in red, revealing a single band at about 50kDa present in all lanes, though at much lower levels in the tissue lysates.

Immunocytochemistry/Immunofluorescence: Park7(DJ-1) Antibody (4H4) [NBP1-92715] - HeLa cells stained with NBP1-92715 (green), chicken antibody to Vimentin (NB300-223, red) and DNA (blue). NBP1-92715 reveals strong cytoplasmic staining for Park7(DJ-1).

Simple Western: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Lane view shows a specific band for Park7 (DJ-1) in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Bv, Mu(-), Rt(-)
• Applications: WB, Simple Western, ICC/IF, IHC, KO
• Clone: 4H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Park7/DJ-1 Antibody (4H4) - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse Park7/DJ-1 Antibody (4H4) - BSA Free (NBP1-92715) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-Park7/DJ-1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length recombinant human Park7(DJ-1) expressed in and purified from E. coli. [UniProt# Q99497]
• Localization: Cytoplasm. Nucleus. Mitochondrion. Note: Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PARK7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:5000
  • Immunohistochemistry 1:5000
  • Knockout Validated
  • Simple Western 1:500
  • Western Blot 1:5000
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:500, apparent MW was 26 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Not reactive with rodent.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Park7/DJ-1 Antibody (4H4) - BSA Free

• DJ1
• DJ-1
• DJ1FLJ34360
• EC 3.4
• FLJ27376
• FLJ92274
• Oncogene DJ1
• Park7
• Parkinson disease (autosomal recessive, early onset) 7
• Parkinson disease protein 7
• protein DJ-1

Background

Park7(DJ-1) (Parkinson disease protein 7/ oncogene DJ1, also known as CAP1) was initially discovered as an oncogene promoting Ras-dependent transformation and later it was identified as being responsible for an autosomal recessive early-onset form of Parkinson's disease type 7 (PARK7). Member of peptidase C56 family, Park7(DJ-1) exists as homodimer located predominantly in the cytoplasm to a lesser extent in nucleus as well as mitochondrion, and besides being an essential part of a ternary complex containing PARK7, EFCAB6/DJBP and AR, it interacts with OTUD7B, BBS1, HIPK1, CLCF1, MTERF, EFCAB6/DJBP and PIAS2. Park7(DJ-1) is an evolutionary conserved ubiquitously expressed multifunctional protein involved in several biological processes including transcriptional regulation, oxidative stress, carcinogenesis, cell growth and transformation, AR-dependent transcription regulation, apoptosis, inflammation, redox-sensitive chaperon activities, protein degradation, fertilization and regulation of mitochondrial morphology /function as well as for autophagy of dysfunctional mitochondria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Park7/DJ-1 Antibody (NBP1-92715)(2)

Publications using NBP1-92715

• Repici M, Hassanjani M, Maddison DC et al. The Parkinson's Disease-Linked Protein DJ-1 Associates with Cytoplasmic mRNP Granules During Stress and Neurodegeneration. Mol Neurobiol 2018-04-19 [PMID: 29675578]
• Wang C, Fang M, Zhang M et al. The positive correlation between DJ-1 and beta-catenin expression shows prognostic value for patients with glioma. Neuropathology 2013-05-28 [PMID: 23714193] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: PARK7
• Entrez:
  • 11315(Human)
• Uniprot:
  • Q99497()