Pappalysin-1/PAPP-A Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Pappalysin-1/PAPP-A Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Pappalysin-1/PAPP-A (R&D Systems, Catalog # 2487-ZN)
  Glu82-Asp1214
  Accession # Q13219
• Specificity: Detects human Pappalysin-1/PAPP-A in Western blots. In this format, less than 5% cross-reactivity with recombinant human PAPP‑A2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PAPPA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pappalysin-1/PAPP-A Antibody [Biotin]

• ASBABP2
• DIPLA1
• EC 3.4.24.79
• IGFBP-4ase
• IGFBP-4aseaspecific BCL2 ARE-binding protein 2
• IGF-dependent IGFBP-4 protease
• insulin-like growth factor-dependent IGF binding protein-4 protease
• Insulin-like growth factor-dependent IGF-binding protein 4 protease
• PAPA
• PAPPA
• PAPPA1
• PAPP-A1
• PAPP-Adifferentially placenta 1 expressed protein
• Pappalysin1
• Pappalysin-1
• pregnacy-associated plasma protein A
• Pregnancy-associated plasma protein A
• pregnancy-associated plasma protein A, pappalysin 1

Background

Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP-A are significantly reduced when a fetus affected by Down syndrome is present (2). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (3). Lack of IGFBP-4 cleavage in embryonic fibroblasts derived from PAPP-A knockout mice indicates that PAPP-A functions as a physiological IGFBP-4 protease (4). Three Lin12-Notch repeats (LNR) in the PAPP-A protein bind Ca²⁺ and are required for the cleavage of IGFBP-4, not IGFBP-5, by PAPP-A (5). The C-terminal LNR (residues 1476 to 1503) is not present in rhPAPP-A (residues 82 to 1214), which starts at the N-terminus of the mature chain and ends before the five Sushi (SCR) modules. As an active protease, rhPAPP-A cleaves IGFBP-5, which can be inhibited by 1,10-phenanthroline.

1. Boldt, H.B. et al. (2001) Biochem. J. 358:359.
2. Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
3. Laursen, L.S. et al. (2001) FEBS Lett. 504:36.
4. Conover, C.A. et al. (2004) Development 131:1187.
5. Boldt, H.B. et al. (2004) J. Biol. Chem. 279:38525.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Pappalysin-1/PAPP-A Antibody (BAF2487). (Showing 1 - 1 of 1 FAQ).

1. I am highly interested to use couple of Anti-PAPP-A antibodies from your reputed companies for my research work. I have got the information regarding the immunogen used to generate these antibodies, however could not get any information regarding the specific amino acids/epitope to which your Anti-PAPP-A Antibodies binds with the antigen (PAPP-A).
   • Unfortunately, none of our PAPP A antibodies have been epitope mapped. Many of them have also been generated against full-length PAPP A, so there are many potential binding sites.

Bioinformatics

• Gene Symbol: PAPPA
• Uniprot:
  • Q13219()