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Galectin 13 Antibody (PP13/1161)

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Western Blot: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Western Blot of (A) Recombinant human Galectin-13 fragment And (B) K562 Cell Lysate using Galeactin-13 MAb (PP13/1161).
Western Blot: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Western Blot of (A) Recombinant human Galectin-13 fragment And (B) K562 Cell Lysate using Galeactin-13 MAb (PP13/1161).
Immunohistochemistry-Paraffin: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Formalin-paraffin human Spleen stained with Galectin-13MAb (PP13/1161).
Immunohistochemistry-Paraffin: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Formalin-paraffin human Placenta stained with Galectin-13 MAb (PP13/1161).
SDS-Page: Galectin 13 Antibody (PP13/1161) [NBP2-53210]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-P, PAGE
Clone
PP13/1161
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-54358AF405
Alexa Fluor 488
NBP2-54358AF488
Alexa Fluor 647
NBP2-54358AF647
Alexa Fluor 700
NBP2-54358AF700
Allophycocyanin
NBP2-54358APC
Biotin
NBP2-54358B
DyLight 350
NBP2-54358UV
DyLight 405
NBP2-54358V
DyLight 550
NBP2-54358R
DyLight 650
NBP2-54358C
DyLight 680
NBP2-54358FR
DyLight 755
NBP2-54358IR
FITC
NBP2-54358F
HRP
NBP2-54358H
Janelia Fluor 549
NBP2-54358JF549
Janelia Fluor 646
NBP2-54358JF646
PE
NBP2-54358PE
PerCP
NBP2-54358PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-54358-100ug
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Galectin 13 Antibody (PP13/1161) Summary

Immunogen
Recombinant human Galectin-13 protein fragment (aa23-134) (exact sequence is proprietary)
Localization
Cytoplasmic & Cell Surface
Protein/Peptide Type
Recombinant Protein
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LGALS13
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5 - 1 ug/ml
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • SDS-Page
Application Notes
ELISA (For coating order Ab without BSA)
IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA buffer pH 9.0 or 10mM Citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
1mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Galectin 13 Antibody (PP13/1161)

  • beta-galactoside-binding lectin
  • EC 3.1.1.5
  • GAL13
  • gal-13
  • galectin-13
  • lectin, galactoside-binding, soluble, 13
  • PLAC8galectin 13
  • Placental protein 13
  • Placental tissue protein 13
  • PP13galactoside-binding soluble lectin 13

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Galectin‑1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Galectin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 1.7 µg/mL Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Species: Hu
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
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Antibody (A3A12)
NB300-538
Western Blot: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of 25 ug of MCF-7 (Lane 1), Hela (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.
Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - LLOMe treatment results in lysosomal damage in T98G glioblastoma cells as evidenced by the accumulation of galectin-3 positive vesicles. T98G cells were treated with LLOMe (1 uM) for 1 h to induce lysosomal damage. Cells were fixed (4% paraformaldehyde) 6 h after washout, blocked with 10% goat serum in 0.1% PBST (1 h, RT), stained with DyLight 488 conjugated galectin-3 antibody (green) (overnight, 4C).The following day, samples were washed with cold PBST, mounted using Prolong Gold mountant with DAPI (blue). Confocal immunofluorescence using DyLight 488 conjugated galectin-3 antibody. This image was submitted via customer Review. Image using the DyLight 488 form of this antibody.
Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Human colon tissue (right) compared with a negative control in the absence of primary antibody (left).
(2 Publications)
Species: Hu, Mu, Rt, Rb
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Pappalysin-1/PAPP-A An ...
AF2487
Western blot shows lysate of human pregnant sera. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Pappalysin‑1/PAPP‑A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2487) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pappalysin‑1/PAPP‑A at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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ADAM12 Antibody
NB300-889
Immunocytochemistry/Immunofluorescence: ADAM12 Antibody [NB300-889] - analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/plasma membrane staining. The nuclear
Immunohistochemistry-Paraffin: ADAM12 Antibody [NB300-889] - (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: ADAM12 Antibody [NB300-889] - analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) f
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Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Endoglin/CD105
Antibody [Unconjugate
AF1097
Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
Endoglin/CD105 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Galectin-10
Antibody
NBP1-87688
Western Blot: Galectin-10 Antibody [NBP1-87688] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HL-60
Immunohistochemistry-Paraffin: Galectin-10 Antibody [NBP1-87688] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: Galectin-10 Antibody [NBP1-87688] - Staining of human bone marrow shows strong cytoplasmic positivity in subsets of hematopoietic cells.
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AKR1B1 Antibody
NBP1-89146
Western Blot: AKR1B1 Antibody [NBP1-89146] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody [NBP1-89146] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining in human adrenal gland and cerebral cortex tissues using anti-AKR1B1 antibody. Corresponding AKR1B1 RNA-seq data are presented for the same tissues.
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Applications: WB, ICC/IF, IHC, IHC-P
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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Prolyl
Oligopeptidase/PREP A
NBP2-33950
Western Blot: Prolyl Oligopeptidase/PREP Antibody [NBP2-33950] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: Prolyl Oligopeptidase/PREP Antibody [NBP2-33950] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody stainin is shown in green.
Immunohistochemistry-Paraffin: Prolyl Oligopeptidase/PREP Antibody [NBP2-33950] - Staining in human colon and pancreas tissues using anti-PREP antibody. Corresponding PREP RNA-seq data are presented for the same tissues.
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Applications: WB, ICC/IF, IHC, IHC-P
HLA DQA1 Antibody
NBP1-84550
Western Blot: HLA DQA1 Antibody [NBP1-84550] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line Daudi.
Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human lung shows high expression.
Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
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CTLA-4
7268-CT
CTLA-4
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AP3B1 Antibody
NBP1-89228
Immunocytochemistry/Immunofluorescence: AP3B1 Antibody [NBP1-89228] - Staining of human cell line HeLa shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.
Species: Hu
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PIGF Antibody
NBP1-69261
Western Blot: PIGF Antibody [NBP1-69261] - This Anti-PIGF antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 1ug/ml.
Immunohistochemistry: PIGF Antibody [NBP1-69261] - Human Bronchial Epithelial Tissue Observed Staining: Cytoplasm in Human Bronchial Epithelial Tissue Primary Antibody Concentration: 1 : 100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X.
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COX-2 Antibody
NB100-689
Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody.
Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded human colon carcinoma stained with COX2 antibody.
Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded human colon carcinoma stained with COX2 antibody.
(1 Review)
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Glut1 Antibody
NB110-39113
Western Blot: Glut1 Antibody [NB110-39113] - Analysis of GLUT1 on A. mouse kidney membrane protein and B. rat kidney membrane protein.
Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).
Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.
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PLA2G4A Antibody
NBP2-19809
Western Blot: Phospholipase A2, group IVA Antibody [NBP2-19809] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:3000.
Immunocytochemistry/Immunofluorescence: Phospholipase A2, group IVA Antibody [NBP2-19809] - Sample: HeLa cells were fixed in iced-cold MeOH for 5 min. Green: PLA2G4A protein stained by PLA2G4A antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Phospholipase A2, group IVA Antibody [NBP2-19809] - Immunohistochemical analysis of paraffin-embedded Lung ca, using antibody at 1:500 dilution.
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Applications: WB, ICC/IF, IHC, IHC-P
Phospholipase B1
Overexpression Lysate ...
H00151056-T01
Lane 1: PLB1 transfected lysate (46.64 KDa) Lane 2: Non-transfected lysate.
PLB1 transfected lysate.
Species: Hu
Galectin 13
Antibody (PP13/1161)
NBP2-53210
Galectin 13 Antibody (PP13/1161)
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-P, PAGE

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Bioinformatics

Gene Symbol LGALS13