PA2G4/EBP1 Antibody

Images

 
Western Blot: EBP1 Antibody [NBP2-16257] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: EBP1 Antibody [NBP2-16257] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Western Blot: EBP1 Antibody [NBP2-16257] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.55 mg/ml

Order Details

PA2G4/EBP1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EBP1. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus; nucleolus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PA2G4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (97%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.55 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PA2G4/EBP1 Antibody

  • Cell cycle protein p38-2G4 homolog
  • EBP1
  • ErbB-3 binding protein 1
  • ErbB3-binding protein 1
  • ErbB3-binding protein Ebp1
  • HG4-1
  • p38-2G4
  • PA2G4
  • proliferation-associated 2G4, 38kD
  • proliferation-associated 2G4, 38kDa
  • proliferation-associated protein 2G4

Background

This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PA2G4
Entrez
Uniprot