CRLF3 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: CRLF3 Antibody [NBP1-84382] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.

Western Blot: CRLF3 Antibody [NBP1-84382] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Immunohistochemistry-Paraffin: CRLF3 Antibody [NBP1-84382] - Staining of human lymph node shows high expression.

Immunohistochemistry-Paraffin: CRLF3 Antibody [NBP1-84382] - Staining of human pancreas shows low expression as expected.

Analysis in human lymph node and pancreas tissues using Anti-CRLF3 antibody. Corresponding CRLF3 RNA-seq data are presented for the same tissues.

Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CRLF3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CRLF3 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: IEHGVNTAEDLVREGEIAMLGGVGEENEKLWSFTKKASHIQLDSLPEVPLLVDVPCLSAQLDDSILNIVKDHIFKHGTVASRPPVQIEELIEKPGGIIVRWCKVDDDFTAQDYRLQFRKCTSNHFEDVYVGSE
• Predicted Species: Mouse (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRLF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CRLF3 Protein (NBP1-84382PEP)

Reactivity Notes

Rat (89%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CRLF3 Antibody - BSA Free

• CREME-9
• CREME9MGC20661
• cytokine receptor-like factor 3
• Cytokine receptor-like molecule 9
• Cytokine receptor-related protein 4
• CYTOR4cytokine receptor related protein 4
• FRWS
• P48
• p48.2
• type I cytokine receptor like factor
• Type I cytokine receptor-like factor p48

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRLF3